Anti-Annexin A3/ANXA3 antibody (ab127924)
Key features and details
- Rabbit polyclonal to Annexin A3/ANXA3
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Annexin A3/ANXA3 antibody
See all Annexin A3/ANXA3 primary antibodies -
Description
Rabbit polyclonal to Annexin A3/ANXA3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Rabbit, Horse -
Immunogen
Synthetic peptide corresponding to Rat Annexin A3/ANXA3 aa 124-141 (internal sequence).
Sequence:RQMKEISQAYYTAYKKNL
Database link: P14669 -
Positive control
- Rat brain, lung, testis and skeletal muscle lysates; MCF7, HeLa and SW620 cell lysates; Human mammary cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab127924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa.
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Inhibitor of phospholipase A2, also possesses anti-coagulant properties. Also cleaves the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid. - Information by UniProt
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Database links
- Entrez Gene: 306 Human
- Entrez Gene: 25291 Rat
- Omim: 106490 Human
- SwissProt: P12429 Human
- SwissProt: P14669 Rat
- Unigene: 480042 Human
- Unigene: 6589 Rat
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Alternative names
- 2-cyclic phosphate 2-phosphohydrolase antibody
- 35-alpha calcimedin antibody
- Annexin A3 antibody
see all
Images
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All lanes : Anti-Annexin A3/ANXA3 antibody (ab127924) at 1 µg/ml
Lane 1 : Rat Brain Tissue Lysate
Lane 2 : Rat Lung Tissue Lysate
Lane 3 : Rat Testicular Tissue Lysate
Lane 4 : Rat Skeletal Muscle Tissue Lysate
Lane 5 : Rabbit IgG (55KD)
Lane 6 : MCF7 Cell Lysate
Lane 7 : HeLa Cell Lysate
Lane 8 : SW620 Cell Lysate
Predicted band size: 36 kDa -
ab127924 at 1µg/ml staining Annexin A3/ANXA3 in Paraffin-embedded Human Mammary Cancer Tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab127924 has been referenced in 3 publications.
- Wang Y et al. ANXA3 Silencing Ameliorates Intracranial Aneurysm via Inhibition of the JNK Signaling Pathway. Mol Ther Nucleic Acids 17:540-550 (2019). PubMed: 31362241
- Zhou T et al. Annexin A3 as a Prognostic Biomarker for Breast Cancer: A Retrospective Study. Biomed Res Int 2017:2603685 (2017). IHC ; Human . PubMed: 28497041
- Tome ME et al. Identification of P-glycoprotein co-fractionating proteins and specific binding partners in rat brain microvessels. J Neurochem 134:200-10 (2015). PubMed: 25832806