Product nameAnti-Annexin A3 antibody
See all Annexin A3 primary antibodies
DescriptionRabbit polyclonal to Annexin A3
Tested applicationsSuitable for: ELISA, WB, IHC-P, ICC/IF, IHC - Wholemountmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse
- WB: Placenta lysate. IHC-P: Human spleen tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab33068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. Used at a dilution of 1/100 for 1 hr incubation on mouse cartilage (see Abreview for further information).|
|IHC - Wholemount||Use at an assay dependent concentration.|
FunctionInhibitor of phospholipase A2, also possesses anti-coagulant properties. Also cleaves the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate.
Sequence similaritiesBelongs to the annexin family.
Contains 4 annexin repeats.
DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
- Information by UniProt
- 2-cyclic phosphate 2-phosphohydrolase antibody
- 35-alpha calcimedin antibody
- Annexin A3 antibody
Anti-Annexin A3 antibody (ab33068) at 1.25 µg/ml + placenta lysate at 10 µg
HRP conjugated anti Rabbit IgG at 1/50000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling Annexin A3 with ab33068 at 4µg/ml. Positively labelled cells indicated by arrows. Magnification: 400X.
This product has been referenced in:
- Subhasitanont P et al. Apigenin inhibits growth and induces apoptosis in human cholangiocarcinoma cells. Oncol Lett 14:4361-4371 (2017). Read more (PubMed: 28943950) »
- Qu S et al. Protein expression of nucleophosmin, annexin A3 and nm23-H1 correlates with human nasopharyngeal carcinoma radioresistance in vivo. Oncol Lett 12:615-620 (2016). IHC-P ; Human . Read more (PubMed: 27347189) »