Overview

  • Product name
  • Description
    Rabbit polyclonal to Annexin A3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WB, IHC-P, ICC/IF, IHC - Wholemountmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse
  • Immunogen

    A region within synthetic peptide: MASIWVGHRG TVRDYPDFSP SVDAEAIQKA IRGIGTDEKM LISILTERSN, corresponding to N terminal amino acids 1-50 of Human Annexin A3

  • Positive control
    • WB: Placenta lysate. IHC-P: Human spleen tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab33068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/62500.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. Used at a dilution of 1/100 for 1 hr incubation on mouse cartilage (see Abreview for further information).
IHC - Wholemount Use at an assay dependent concentration.

Target

  • Function
    Inhibitor of phospholipase A2, also possesses anti-coagulant properties. Also cleaves the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate.
  • Sequence similarities
    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2-cyclic phosphate 2-phosphohydrolase antibody
    • 35-alpha calcimedin antibody
    • Annexin A3 antibody
    • Annexin III antibody
    • Annexin-3 antibody
    • ANX3 antibody
    • ANXA 3 antibody
    • Anxa3 antibody
    • ANXA3_HUMAN antibody
    • Calcimedin 35 alpha antibody
    • Inositol 1 2 cyclic phosphate 2 phosphohydrolase antibody
    • Inositol 1 antibody
    • Lipocortin III antibody
    • OTTHUMP00000160701 antibody
    • OTTHUMP00000219237 antibody
    • OTTHUMP00000219238 antibody
    • PAP III antibody
    • PAP-III antibody
    • Placental anticoagulant protein III antibody
    see all

Images

  • Anti-Annexin A3 antibody (ab33068) at 1.25 µg/ml + placenta lysate at 10 µg

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa
    Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling Annexin A3 with ab33068 at 4µg/ml. Positively labelled cells indicated by arrows. Magnification: 400X.

References

This product has been referenced in:
  • Subhasitanont P  et al. Apigenin inhibits growth and induces apoptosis in human cholangiocarcinoma cells. Oncol Lett 14:4361-4371 (2017). Read more (PubMed: 28943950) »
  • Qu S  et al. Protein expression of nucleophosmin, annexin A3 and nm23-H1 correlates with human nasopharyngeal carcinoma radioresistance in vivo. Oncol Lett 12:615-620 (2016). IHC-P ; Human . Read more (PubMed: 27347189) »
See all 6 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (placenta)
Total protein in input
3600 µg
Immuno-precipitation step
Protein A
Specification
placenta

Abcam user community

Verified customer

Submitted Oct 02 2015

Application
IHC - Wholemount
Sample
Mouse Embryo (Whole mouse embryo)
Specification
Whole mouse embryo

Abcam user community

Verified customer

Submitted Feb 24 2012

Answer

DISCOUNT CODE: xx

Expiration date: 14. Juin 2012

I am very pleased to hear you would like to accept our offer and test ab33068 in mouse sections. This code will give you 1 free primary antibody before the expiration date. The code will be activated in both cases, if the antibody does not work and as well if the antibody is working. All we need form you toredeem this offer, is that you willsubmit an Abreviewfor ab33068 with the results on mouse sections. Pleaseinclude this codein the Additional Comments section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

As said, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you very much for your interest in ab30988.

Unfortunately we do not have small aliquots of our products, however we can offer you a testing discount. Indeed, if you would test one of our antibodies in a new species or a new application and submit the feedback, we can offer you a 100% discount on a future antibody purchase. Please see belowfor further information.

The ab30988 has not been tested in immunohistochemistry, nor on mouse. The alignement with the mouse sequence is however quite good, with a score of 87% alignement. http://www.ebi.ac.uk/Tools/services/web/toolresult.ebi?jobId=clustalw2-I20120210-090810-0481-51531125-pg&tool=clustalw2&analysis=alignments
https://www.abcam.com/index.html?datasheet=30988 (or use the following: https://www.abcam.com/index.html?datasheet=30988).

The ab33068 has been tested in immunohistochemistry on paraffin sections and the alignement scorewith the mouse sequence is84% - there are therefore reasonable chances of crossreactivity. http://www.ebi.ac.uk/Tools/services/web/toolresult.ebi?jobId=clustalw2-I20120210-085947-0081-51081439-pg&tool=clustalw2&analysis=alignments
https://www.abcam.com/index.html?datasheet=33068 (or use the following: https://www.abcam.com/index.html?datasheet=33068).

The ab79904 has been tested on mouse tissue, however not in immunohistochemistry. It has been tested in Western blot and in ELISA however. https://www.abcam.com/index.html?datasheet=79904 (or use the following: https://www.abcam.com/index.html?datasheet=79904).

In summary, I think all of these three antibodies have a reasonable chance to work in IHC and mouse, however this needs to be tested. I could provide you with a testing discount on any of these three antibodies, please let me know which one would be most interesting for you.


If you are interested in the testing discount, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test one of these three antibodies in IHC in mouse. I will then send a discount code. This code must be issued before purchasingthe antibodyso please wait for my reply before ordering.

2. Purchasethe antibody either by phone, fax, or online (www.abcam.com).

3. Test it in IHC in mouse.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifthe antibodyturns out to be unsuitable for IHC on mouse sections, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.



.

Read More

Question
Answer

Thank you for your enquiry. Alignment of the immunogens with rat and mouse annexin A3 gives the following results: ab33068, mouse 78% identity, rat 64% identity. ab30988, mouse 92% identity, rat 85% identity. I hope this helps. Please do not hesitate to contact us if you have any other questions.

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse (cartilage)
Specification
cartilage
Fixative
Methanol
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5

Abcam user community

Verified customer

Submitted Jul 13 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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