Product nameAnti-Annexin V antibody
See all Annexin V primary antibodies
DescriptionRabbit polyclonal to Annexin V
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Chimpanzee
- HepG2 cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab30941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Involvement in diseasePregnancy loss, recurrent, 3
Sequence similaritiesBelongs to the annexin family.
Contains 4 annexin repeats.
DomainThe [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid.
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
- Information by UniProt
- Anchorin CII antibody
- Annexin 5 antibody
- Annexin A5 antibody
Anti-Annexin V antibody (ab30941) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 36 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Gel concentration: 12%
This product has been referenced in:
- Stopa JD et al. Kinetic-based trapping by intervening sequence variants of the active sites of protein-disulfide isomerase identifies platelet protein substrates. J Biol Chem 292:9063-9074 (2017). Read more (PubMed: 28364042) »
- Grzendowski M et al. Differential proteome analysis of human gliomas stratified for loss of heterozygosity on chromosomal arms 1p and 19q. Neuro Oncol 12:243-56 (2010). WB ; Human . Read more (PubMed: 20167812) »