Validated using a knockout cell line

Anti-Annexin V antibody [EPR3980] (ab108194)


  • Product name
    Anti-Annexin V antibody [EPR3980]
    See all Annexin V primary antibodies
  • Description
    Rabbit monoclonal [EPR3980] to Annexin V
  • Host species
  • Specificity
    The immunogen used for this product shares 80% homology with ANXA2 and 73% homology with ANXA4. Cross-reactivity with this protein has not been confirmed experimentally.
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Annexin V aa 300 to the C-terminus. The exact sequence is proprietary.

  • Positive control
    • WB: HeLa, fetal kidney, fetal brain, Jurkat and JAR cell lysates ICC: HeLa cells; staurosporin-treated apoptotic HeLa cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab108194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 36 kDa.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/500 - 1/1000.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
    • Involvement in disease
      Pregnancy loss, recurrent, 3
    • Sequence similarities
      Belongs to the annexin family.
      Contains 4 annexin repeats.
    • Domain
      The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
      A pair of annexin repeats may form one binding site for calcium and phospholipid.
    • Post-translational
      S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
    • Information by UniProt
    • Database links
    • Alternative names
      • Anchorin CII antibody
      • Annexin 5 antibody
      • Annexin A5 antibody
      • Annexin V antibody
      • Annexin-5 antibody
      • ANX A5 antibody
      • ANX5 antibody
      • ANXA5 antibody
      • ANXA5_HUMAN antibody
      • Calphobindin I antibody
      • CBP-I antibody
      • Endonexin II antibody
      • ENX2 antibody
      • Lipocortin V antibody
      • PAP I antibody
      • PAP-I antibody
      • Placental anticoagulant protein 4 antibody
      • Placental anticoagulant protein I antibody
      • PLACENTAL PROTEIN 4 antibody
      • Pp4 antibody
      • Thromboplastin inhibitor antibody
      • VAC-alpha antibody
      • Vascular anticoagulant-alpha antibody
      see all


    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: Annexin V knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)

      Lanes 1 - 3: Merged signal (red and green). Green - ab108194 observed at 35 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab108194 was shown to recognize Annexin V in wild-type cells as signal was lost at the expected MW in Annexin V knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Annexin V knockout samples were subjected to SDS-PAGE. Ab108194 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Overlay histogram showing HeLa cells stained with ab108194 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108194, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-Annexin V antibody [EPR3980] (ab108194)

      Lane 1 : HeLa cell lysate
      Lane 2 : Fetal kidney lysate
      Lane 3 : Fetal brain lysate
      Lane 4 : Jurkat cell lysate
      Lane 5 : JAR cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 36 kDa

    • Immunofluorescent staining of HeLa cells using 1/500 ab108194.
    • Immunofluorescent staining of staurosporin-treated apoptotic HeLa cells using 1/250 ab108194.


    This product has been referenced in:
    • Middleton RC  et al. Newt cells secrete extracellular vesicles with therapeutic bioactivity in mammalian cardiomyocytes. J Extracell Vesicles 7:1456888 (2018). Read more (PubMed: 29696078) »
    • Ververis K & Karagiannis TC An atlas of histone deacetylase expression in breast cancer: fluorescence methodology for comparative semi-quantitative analysis. Am J Transl Res 4:24-43 (2012). ICC/IF ; Human . Read more (PubMed: 22347520) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Thank you for contacting us and for your patience.

    I have contacted the originator of anti-Annexin V antibody ab54775 and our other anti-Annexin V antibodies:

    Unfortunately, it is not known whether ab54775 is capable of blocking Ann...

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