Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader, Flow cytometer
- Sample type: Suspension cells
Product nameAnnexin V-APC Assay Kit
See all Annexin V/ANXA5 kits
Sample typeSuspension cells
Assay typeCell-based (quantitative)
Species reactivityReacts with: Human
Annexin V-APC Assay Kit (ab236215) employs an APC-conjugated Annexin V as a probe for phosphatidylserine on the outer membrane of apoptotic cells. DAPI is used as a marker of cell membrane permeability seen in very late apoptotic or necrotic cells. The reagents provided are sufficient to run 100 samples when using a 96-well format.
PlatformMicroplate reader, Flow cytometer
Storage instructionsStore at +4°C. Please refer to protocols.
Components 100 tests Annexin V APC Assay Reagent 1 x 100 tests Cell-Based Assay Annexin V Binding Buffer (10X) 1 x 50ml DAPI Viability Dye 1 x 100µl
FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Involvement in diseasePregnancy loss, recurrent, 3
Sequence similaritiesBelongs to the annexin family.
Contains 4 annexin repeats.
DomainThe [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid.
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
- Information by UniProt
- Anchorin CII
- Annexin 5
- Annexin A5
Jurkat (Human T cell leukemia cell line from peripheral blood) cells were left untreated (panel A) or stimulated with 200 mJ/cm2 ultraviolet light (panel B) and then incubated for 4 hours at 37°C. Cells were stained using Annexin V APC Assay Reagent at a 1:200 dilution in 1X Binding Buffer for 15 minutes at RT in the dark. After centrifugation and removal of the supernatant, cells were resuspended in 1:100 DAPI in PBS.
ab236215 has been referenced in 1 publication.
- Kang DW et al. Phospholipase D1 inhibition sensitizes glioblastoma to temozolomide and suppresses its tumorigenicity. J Pathol 252:304-316 (2020). PubMed: 32725633