Overview

  • Product name

    Annexin V-APC Assay Kit
    See all Annexin V/ANXA5 kits
  • Detection method

    Fluorescent
  • Sample type

    Suspension cells
  • Assay type

    Cell-based (quantitative)
  • Species reactivity

    Reacts with: Human
  • Product overview

    Annexin V-APC Assay Kit (ab236215) employs an APC-conjugated Annexin V as a probe for phosphatidylserine on the outer membrane of apoptotic cells. DAPI is used as a marker of cell membrane permeability seen in very late apoptotic or necrotic cells. The reagents provided are sufficient to run 100 samples when using a 96-well format.

  • Platform

    Microplate reader, Flow cytometer

Properties

Images

  • Jurkat (Human T cell leukemia cell line from peripheral blood) cells were left untreated (panel A) or stimulated with 200 mJ/cm2 ultraviolet light (panel B) and then incubated for 4 hours at 37°C. Cells were stained using Annexin V APC Assay Reagent at a 1:200 dilution in 1X Binding Buffer for 15 minutes at RT in the dark. After centrifugation and removal of the supernatant, cells were resuspended in 1:100 DAPI in PBS.

Protocols

References

ab236215 has not yet been referenced specifically in any publications.

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