Annexin V-CF Blue 7-AAD Apoptosis Staining / Detection Kit (ab214663)

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Abcam’s Apoptosis Detection (Annexin V-CF Blue 7-AAD) Kit Jurkat cells (T-cell leukemia, human) treated with 6 µM camptothecin for four hours.
  • Abcam’s Apoptosis Detection (Annexin V-CF Blue 7-AAD) Kit ab214663 Jurkat cells (T-cell leukemia, human) untreated.

Key features and details

  • Assay type: Cell-based (quantitative)
  • Detection method: Fluorescent
  • Platform: Flow cytometer
  • Sample type: Adherent cells, Suspension cells

Overview

  • Product name

    Annexin V-CF Blue 7-AAD Apoptosis Staining / Detection Kit
    See all Annexin V/ANXA5 kits

  • Detection method

    Fluorescent

  • Sample type

    Adherent cells, Suspension cells

  • Assay type

    Cell-based (quantitative)

  • Species reactivity

    Reacts with: Mammals, Other species

  • Product overview

    Annexin V-CF Blue 7-AAD Apoptosis Staining / Detection Kit (ab214663) contains Annexin V labeled with CF Blue, which allows the identification and quantitation of apoptotic cells on a single-cell basis by flow cytometry. Simultaneous staining of cells with Annexin V–CF Blue (blue fluorescence) and the non-vital dye 7-aminoactinomycin D (7-AAD) (red fluorescence) allows the discrimination of intact cells (Annexin V-CF Blue negative, 7-AAD Staining Solution negative), early apoptotic (Annexin V-CF Blue positive, 7-AAD Staining Solution negative) and late apoptotic or necrotic cells (Annexin V-CF Blue positive, 7-AAD Staining Solution positive).


    CF-Blue can be used as direct replacement for Pacific Blue™ or similar blue dyes.


    For more apoptosis assays, review the full set of Annexin V assays, or the apoptosis assay and apoptosis marker guide.

  • Notes

    Exposure of phosphatidylserine (PS) on the external surface of the cell membrane has been reported to occur in the early phases of apoptotic cell death, during which the cell membrane remains intact. In leukocyte apoptosis, PS on the outer surface of the cell marks the cell for recognition and phagocytosis by macrophages. The Annexin V protein has a high affinity for PS, and shows minimal binding to phosphatidylcholine and sphingomyelin. Changes in PS asymmetry, which can be analyzed by measuring annexin V binding to the cell membrane, are generally observed before morphological changes associated with apoptosis occurred and before membrane integrity is lost.

  • Platform

    Flow cytometer

Properties

Images

  • Abcam’s Apoptosis Detection (Annexin V-CF Blue 7-AAD) Kit Jurkat cells (T-cell leukemia, human) treated with 6 µM camptothecin for four hours.
    Abcam’s Apoptosis Detection (Annexin V-CF Blue 7-AAD) Kit Jurkat cells (T-cell leukemia, human) treated with 6 µM camptothecin for four hours.

    Abcam’s Apoptosis Detection (Annexin V-CF Blue 7-AAD) Kit Jurkat cells (T-cell leukemia, human) treated with 6 μM camptothecin for four hours.

  • Abcam’s Apoptosis Detection (Annexin V-CF Blue 7-AAD) Kit ab214663 Jurkat cells (T-cell leukemia, human) untreated.
    Abcam’s Apoptosis Detection (Annexin V-CF Blue 7-AAD) Kit ab214663 Jurkat cells (T-cell leukemia, human) untreated.

    Abcam’s Apoptosis Detection (Annexin V-CF Blue 7-AAD) Kit ab214663 Jurkat cells (T-cell leukemia, human) untreated.

References (5)

Publishing research using ab214663? Please let us know so that we can cite the reference in this datasheet.

ab214663 has been referenced in 5 publications.

  • Wong N  et al. K-means quantization for a web-based open-source flow cytometry analysis platform. Sci Rep 11:6735 (2021). PubMed: 33762594
  • Petragnano F  et al. Modulating the dose-rate differently affects the responsiveness of human epithelial prostate- and mesenchymal rhabdomyosarcoma-cancer cell line to radiation. Int J Radiat Biol 96:823-835 (2020). PubMed: 32149569
  • Martin-Cofreces NB  et al. The chaperonin CCT controls T cell receptor-driven 3D configuration of centrioles. Sci Adv 6:N/A (2020). PubMed: 33268369
  • de Moura Escobar SJ  et al. Cytotoxic effects of 4'-hydroxychalcone on human neuroblastoma cells (SH-SY5Y). Toxicol In Vitro 61:104640 (2019). PubMed: 31493544
  • Valencia AM  et al. Recurrent SMARCB1 Mutations Reveal a Nucleosome Acidic Patch Interaction Site That Potentiates mSWI/SNF Complex Chromatin Remodeling. Cell 179:1342-1356.e23 (2019). PubMed: 31759698

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