Annexin V-CF Blue 7-AAD Apoptosis Staining / Detection Kit (ab214663)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Fluorescent
- Platform: Flow cytometer
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Annexin V-CF Blue 7-AAD Apoptosis Staining / Detection Kit
See all Annexin V/ANXA5 kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (quantitative) -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Annexin V-CF Blue 7-AAD Apoptosis Staining / Detection Kit (ab214663) contains Annexin V labeled with CF Blue, which allows the identification and quantitation of apoptotic cells on a single-cell basis by flow cytometry. Simultaneous staining of cells with Annexin V–CF Blue (blue fluorescence) and the non-vital dye 7-aminoactinomycin D (7-AAD) (red fluorescence) allows the discrimination of intact cells (Annexin V-CF Blue negative, 7-AAD Staining Solution negative), early apoptotic (Annexin V-CF Blue positive, 7-AAD Staining Solution negative) and late apoptotic or necrotic cells (Annexin V-CF Blue positive, 7-AAD Staining Solution positive).
CF-Blue can be used as direct replacement for Pacific Blue™ or similar blue dyes.
For more apoptosis assays, review the full set of Annexin V assays, or the apoptosis assay and apoptosis marker guide.
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Notes
Exposure of phosphatidylserine (PS) on the external surface of the cell membrane has been reported to occur in the early phases of apoptotic cell death, during which the cell membrane remains intact. In leukocyte apoptosis, PS on the outer surface of the cell marks the cell for recognition and phagocytosis by macrophages. The Annexin V protein has a high affinity for PS, and shows minimal binding to phosphatidylcholine and sphingomyelin. Changes in PS asymmetry, which can be analyzed by measuring annexin V binding to the cell membrane, are generally observed before morphological changes associated with apoptosis occurred and before membrane integrity is lost.
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Platform
Flow cytometer
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 100 tests 10X Binding Buffer Concentrate 1 x 50ml 7-AAD Staining Solution in PBS (pH 7.4) 1 x 500µl Annexin V-CF Blue Conjugate ( PBS, pH 7.2) 1 x 500µl -
Research areas
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Function
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. -
Involvement in disease
Pregnancy loss, recurrent, 3 -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
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Alternative names
- Anchorin CII
- Annexin 5
- Annexin A5
see all
Associated products
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Images
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Abcam’s Apoptosis Detection (Annexin V-CF Blue 7-AAD) Kit Jurkat cells (T-cell leukemia, human) treated with 6 µM camptothecin for four hours.
Abcam’s Apoptosis Detection (Annexin V-CF Blue 7-AAD) Kit Jurkat cells (T-cell leukemia, human) treated with 6 μM camptothecin for four hours.
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Abcam’s Apoptosis Detection (Annexin V-CF Blue 7-AAD) Kit ab214663 Jurkat cells (T-cell leukemia, human) untreated.Abcam’s Apoptosis Detection (Annexin V-CF Blue 7-AAD) Kit ab214663 Jurkat cells (T-cell leukemia, human) untreated.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab214663 has been referenced in 5 publications.
- Wong N et al. K-means quantization for a web-based open-source flow cytometry analysis platform. Sci Rep 11:6735 (2021). PubMed: 33762594
- Petragnano F et al. Modulating the dose-rate differently affects the responsiveness of human epithelial prostate- and mesenchymal rhabdomyosarcoma-cancer cell line to radiation. Int J Radiat Biol 96:823-835 (2020). PubMed: 32149569
- Martin-Cofreces NB et al. The chaperonin CCT controls T cell receptor-driven 3D configuration of centrioles. Sci Adv 6:N/A (2020). PubMed: 33268369
- de Moura Escobar SJ et al. Cytotoxic effects of 4'-hydroxychalcone on human neuroblastoma cells (SH-SY5Y). Toxicol In Vitro 61:104640 (2019). PubMed: 31493544
- Valencia AM et al. Recurrent SMARCB1 Mutations Reveal a Nucleosome Acidic Patch Interaction Site That Potentiates mSWI/SNF Complex Chromatin Remodeling. Cell 179:1342-1356.e23 (2019). PubMed: 31759698