Annexin V-CF Blue 7-AAD Apoptosis Staining / Detection Kit (ab214663)

Submit a review Submit a question

Overview

  • Product name

    Annexin V-CF Blue 7-AAD Apoptosis Staining / Detection Kit
    See all Annexin V/ANXA5 kits

  • Detection method

    Fluorescent

  • Sample type

    Adherent cells, Suspension cells

  • Assay type

    Cell-based (quantitative)

  • Species reactivity

    Reacts with: Mammals, Other species

  • Product overview

    Annexin V-CF Blue 7-AAD Apoptosis Staining / Detection Kit (ab214663) contains Annexin V labeled with CF Blue, which allows the identification and quantitation of apoptotic cells on a single-cell basis by flow cytometry. Simultaneous staining of cells with Annexin V–CF Blue (blue fluorescence) and the non-vital dye 7-aminoactinomycin D (7-AAD) (red fluorescence) allows the discrimination of intact cells (Annexin V-CF Blue negative, 7-AAD Staining Solution negative), early apoptotic (Annexin V-CF Blue positive, 7-AAD Staining Solution negative) and late apoptotic or necrotic cells (Annexin V-CF Blue positive, 7-AAD Staining Solution positive).


    CF-Blue can be used as direct replacement for Pacific Blue™ or similar blue dyes.


    For more apoptosis assays, review the full set of Annexin V assays, or the apoptosis assay and apoptosis marker guide.

  • Notes

    Exposure of phosphatidylserine (PS) on the external surface of the cell membrane has been reported to occur in the early phases of apoptotic cell death, during which the cell membrane remains intact. In leukocyte apoptosis, PS on the outer surface of the cell marks the cell for recognition and phagocytosis by macrophages. The Annexin V protein has a high affinity for PS, and shows minimal binding to phosphatidylcholine and sphingomyelin. Changes in PS asymmetry, which can be analyzed by measuring annexin V binding to the cell membrane, are generally observed before morphological changes associated with apoptosis occurred and before membrane integrity is lost.

  • Platform

    Flow cytometer

Properties

Images

  • Abcam’s Apoptosis Detection (Annexin V-CF Blue 7-AAD) Kit Jurkat cells (T-cell leukemia, human) treated with 6 µM camptothecin for four hours.
    Abcam’s Apoptosis Detection (Annexin V-CF Blue 7-AAD) Kit Jurkat cells (T-cell leukemia, human) treated with 6 µM camptothecin for four hours.

    Abcam’s Apoptosis Detection (Annexin V-CF Blue 7-AAD) Kit Jurkat cells (T-cell leukemia, human) treated with 6 μM camptothecin for four hours.

  • Abcam’s Apoptosis Detection (Annexin V-CF Blue 7-AAD) Kit ab214663 Jurkat cells (T-cell leukemia, human) untreated.
    Abcam’s Apoptosis Detection (Annexin V-CF Blue 7-AAD) Kit ab214663 Jurkat cells (T-cell leukemia, human) untreated.

    Abcam’s Apoptosis Detection (Annexin V-CF Blue 7-AAD) Kit ab214663 Jurkat cells (T-cell leukemia, human) untreated.

Protocols

References

ab214663 has not yet been referenced specifically in any publications.

Publishing research using ab214663? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab214663.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up