Annexin V-Cy3 Apoptosis Staining / Detection Kit (ab14142)

Submit a review Q&A (2)References (8)

Key features and details

  • Assay type: Direct
  • Platform: Flow cytometer, Fluorescence microscope
  • Assay time: 10 min
  • Sample type: Adherent cells, Suspension cells

Overview

  • Product name

    Annexin V-Cy3 Apoptosis Staining / Detection Kit
    See all Annexin V/ANXA5 kits

  • Sample type

    Adherent cells, Suspension cells

  • Assay type

    Direct

  • Assay time

    0h 10m

  • Product overview

    Annexin V-Cy3 Apoptosis Staining / Detection Kit ab14142 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.


    The Annexin V-Cy3 reagent contained in the kit is also available as Annexin V-Cy3 reagent ab14143.

  • Notes

    Soon after initiating apoptosis, cells translocate membrane phosphatidylserine molecules from the inner face of the plasma membrane to the cell surface. Phosphatidylserine on the cell surface is detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for phosphatidylserine.

    For more apoptosis assays, review the full set of Annexin V assays, or the apoptosis assay and apoptosis marker guide.

  • Platform

    Flow cytometer, Fluorescence microscope

Properties

References (8)

Publishing research using ab14142? Please let us know so that we can cite the reference in this datasheet.

ab14142 has been referenced in 8 publications.

  • Mahajna S  et al. In Vitro Evaluation of Chemically Analyzed Hypericum Triquetrifolium Extract Efficacy in Apoptosis Induction and Cell Cycle Arrest of the HCT-116 Colon Cancer Cell Line. Molecules 24:N/A (2019). PubMed: 31731693
  • Li W  et al. The role of transcriptional coactivator TAZ in gliomas. Oncotarget 7:82686-82699 (2016). PubMed: 27764783
  • Jadhav SP  et al. microRNA-200b modulates microglia-mediated neuroinflammation via the cJun/MAPK pathway. J Neurochem 130:388-401 (2014). PubMed: 24749688
  • Xu P  et al. The different role of Notch1 and Notch2 in astrocytic gliomas. PLoS One 8:e53654 (2013). Flow Cyt . PubMed: 23349727
  • Zhou X  et al. Downregulation of miR-21 inhibits EGFR pathway and suppresses the growth of human glioblastoma cells independent of PTEN status. Lab Invest 90:144-55 (2010). PubMed: 20048743
  • Honma K  et al. TNFAIP3/A20 functions as a novel tumor suppressor gene in several subtypes of non-Hodgkin lymphomas. Blood 114:2467-75 (2009). PubMed: 19608751
  • Pu P  et al. Downregulation of Wnt2 and beta-catenin by siRNA suppresses malignant glioma cell growth. Cancer Gene Ther : (2008). PubMed: 18949017
  • Saydam O  et al. Herpes simplex virus 1 amplicon vector-mediated siRNA targeting epidermal growth factor receptor inhibits growth of human glioma cells in vivo. Mol Ther 12:803-12 (2005). PubMed: 16112910

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

I would like to know if this kit can be used with paraffin-embedded (IHC-P) or frozen (IHC-Fr) tissues.

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Answer

Thank you for contacting us. This kit can only be used with fresh cells and tissue samples. Any process that leads to membrane damage like fixation and/or freeze-thaw cycles makes the sample incompatible with this kit. So unfortunately it cannot be used with IHC-P. However, we do have quite a few anti-Annexin V antibodies that work well in IHC-P. such as ab14196. https://www.abcam.com/Annexin-V-antibody-ab14196.html I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

I would like to use this kit in IHC on mouse brain. Is it compatible for these purposes?

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Answer

Thank you for your enquiry. Annexin V assay can only be used with fresh tissue sample. Simply incubate annexin V with the tissue piece, then visualize it using confocal microscopy. Frozen or fixed tissue are not suitable for the assay. For frozen or fixed tissue sections, the Active Caspase-3 antibody (ab2302) works well in IHC for both frozen and paraffin sections. I hope this information helps. Please do not hesitate to contact us if you need anything further.

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