Annexin V-Cy3 Apoptosis Staining / Detection Kit (ab14142)
- Datasheet
- References (6)
- Protocols
Overview
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Product nameAnnexin V-Cy3 Apoptosis Staining / Detection Kit
See all Annexin V/ANXA5 kits -
Sample typeAdherent cells, Suspension cells
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Assay typeDirect
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Assay time0h 10m
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Product overview
Annexin V-Cy3 Apoptosis Staining / Detection Kit ab14142 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.
The Annexin V-Cy3 reagent contained in the kit is also available as Annexin V-Cy3 reagent ab14143.
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Notes
Soon after initiating apoptosis, cells translocate membrane phosphatidylserine molecules from the inner face of the plasma membrane to the cell surface. Phosphatidylserine on the cell surface is detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for phosphatidylserine.
For more apoptosis assays, review the full set of Annexin V assays, or the apoptosis assay and apoptosis marker guide.
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PlatformFlow cytometer, Fluorescence microscope
Properties
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Storage instructionsStore at +4°C. Please refer to protocols.
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Components 100 tests Annexin V-Cy3 1 x 500µl Binding Buffer 1 x 50ml -
Research areas
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FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
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Involvement in diseasePregnancy loss, recurrent, 3
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Sequence similaritiesBelongs to the annexin family.
Contains 4 annexin repeats. -
DomainThe [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
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Alternative names
- Anchorin CII
- Annexin 5
- Annexin A5
see all
Associated products
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- Annexin V-FITC Apoptosis Detection Reagent (500X) (ab14082)
- Propidium Iodide (ab14083)
- 10X Annexin V Binding Buffer (ab14084)
- Annexin V-FITC Apoptosis Staining / Detection Kit (ab14085)
- Calcein AM, fluorescent dye for cell viability (ab141420)
- Annexin V-Cy3 Apoptosis Staining / Detection Reagent (ab14143)
- Annexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX (ab14144)
- Annexin V-Cy5 Apoptosis Staining / Detection Reagent (ab14147)
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- Annexin V-PE Apoptosis Staining / Detection Reagent (ab14154)
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- Annexin V-PE-Cy5 Apoptosis Staining / Detection Kit (ab14159)
- Annexin V-Biotin Apoptosis Staining / Detection Reagent (ab14165)
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Protocols
References
This product has been referenced in:
- Li W et al. The role of transcriptional coactivator TAZ in gliomas. Oncotarget 7:82686-82699 (2016). Read more (PubMed: 27764783) »
- Xu P et al. The different role of Notch1 and Notch2 in astrocytic gliomas. PLoS One 8:e53654 (2013). Flow Cyt . Read more (PubMed: 23349727) »
