Annexin V-Cy3 Apoptosis Staining / Detection Kit (ab14142)
Overview
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Product name
Annexin V-Cy3 Apoptosis Staining / Detection Kit
See all Annexin V/ANXA5 kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Direct -
Assay time
0h 10m -
Product overview
Annexin V-Cy3 Apoptosis Staining / Detection Kit ab14142 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.
The Annexin V-Cy3 reagent contained in the kit is also available as Annexin V-Cy3 reagent ab14143.
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Notes
Soon after initiating apoptosis, cells translocate membrane phosphatidylserine molecules from the inner face of the plasma membrane to the cell surface. Phosphatidylserine on the cell surface is detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for phosphatidylserine.
For more apoptosis assays, review the full set of Annexin V assays, or the apoptosis assay and apoptosis marker guide.
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Platform
Flow cytometer, Fluorescence microscope
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 100 tests Annexin V-Cy3 1 x 500µl Binding Buffer 1 x 50ml -
Research areas
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Function
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. -
Involvement in disease
Pregnancy loss, recurrent, 3 -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
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Alternative names
- Anchorin CII
- Annexin 5
- Annexin A5
see all
Associated products
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References
ab14142 has been referenced in 7 publications.
- Li W et al. The role of transcriptional coactivator TAZ in gliomas. Oncotarget 7:82686-82699 (2016). PubMed: 27764783
- Jadhav SP et al. microRNA-200b modulates microglia-mediated neuroinflammation via the cJun/MAPK pathway. J Neurochem 130:388-401 (2014). PubMed: 24749688
- Xu P et al. The different role of Notch1 and Notch2 in astrocytic gliomas. PLoS One 8:e53654 (2013). Flow Cyt . PubMed: 23349727
- Zhou X et al. Downregulation of miR-21 inhibits EGFR pathway and suppresses the growth of human glioblastoma cells independent of PTEN status. Lab Invest 90:144-55 (2010). PubMed: 20048743
- Honma K et al. TNFAIP3/A20 functions as a novel tumor suppressor gene in several subtypes of non-Hodgkin lymphomas. Blood 114:2467-75 (2009). PubMed: 19608751
- Pu P et al. Downregulation of Wnt2 and beta-catenin by siRNA suppresses malignant glioma cell growth. Cancer Gene Ther : (2008). PubMed: 18949017
- Saydam O et al. Herpes simplex virus 1 amplicon vector-mediated siRNA targeting epidermal growth factor receptor inhibits growth of human glioma cells in vivo. Mol Ther 12:803-12 (2005). PubMed: 16112910
