Annexin V-Cy3 Apoptosis Staining / Detection Kit (ab14142)
- Datasheet
- References (6)
- Protocols
Overview
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Product nameAnnexin V-Cy3 Apoptosis Staining / Detection Kit
See all Annexin V kits -
Sample typeAdherent cells, Suspension cells
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Assay typeDirect
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Assay time0h 10m
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Product overview
Abcam's Annexin V-Cy3 Apoptosis Staining / Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidyl-serine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. The one-step staining procedure takes only 10 minutes. Detection can be analyzed by flow cytometry or by fluorescence microscopy.
Visit our FAQs page for tips and troubleshooting. -
Tested applicationsSuitable for: FM, Flow Cytmore details
Properties
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Storage instructionsStore at +4°C. Please refer to protocols.
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Components 100 tests Annexin V-Cy3 1 x 500µl Binding Buffer 1 x 50ml -
Research areas
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FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
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Involvement in diseasePregnancy loss, recurrent, 3
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Sequence similaritiesBelongs to the annexin family.
Contains 4 annexin repeats. -
DomainThe [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
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Alternative names
- Anchorin CII
- Annexin 5
- Annexin A5
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Associated products
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- Annexin V-FITC Apoptosis Detection Reagent (500X) (ab14082)
- Propidium Iodide (ab14083)
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- Annexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX (ab14144)
- Annexin V-Cy5 Apoptosis Staining / Detection Reagent (ab14147)
- Annexin V-Cy5 Apoptosis Staining / Detection Kit (ab14150)
- Annexin V-EGFP Apoptosis Staining / Detection Kit (ab14153)
- Annexin V-PE Apoptosis Staining / Detection Reagent (ab14154)
- Annexin V-PE Apoptosis Staining / Detection Kit (ab14155)
- Annexin V-PE-Cy5 Apoptosis Staining / Detection Kit (ab14159)
- Annexin V-Biotin Apoptosis Staining / Detection Reagent (ab14165)
- Annexin V-Biotin Apoptosis Staining / Detection Kit (ab14190)
- Annexin V Unlabeled Apoptosis Reagent (ab14193)
Applications
Our Abpromise guarantee covers the use of ab14142 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| FM | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent dilution. |
Datasheets and documents
Protocols
References
This product has been referenced in:
- Li W et al. The role of transcriptional coactivator TAZ in gliomas. Oncotarget 7:82686-82699 (2016). Read more (PubMed: 27764783) »
- Xu P et al. The different role of Notch1 and Notch2 in astrocytic gliomas. PLoS One 8:e53654 (2013). Flow Cyt . Read more (PubMed: 23349727) »
