Question (18221) | Annexin V-Cy3 Apoptosis Staining / Detection Kit (ab14142)

Go to datasheet (ab14142)

Question

I would like to use this kit in IHC on mouse brain. Is it compatible for these purposes?

Answer

Thank you for your enquiry. Annexin V assay can only be used with fresh tissue sample. Simply incubate annexin V with the tissue piece, then visualize it using confocal microscopy. Frozen or fixed tissue are not suitable for the assay. For frozen or fixed tissue sections, the Active Caspase-3 antibody (ab2302) works well in IHC for both frozen and paraffin sections. I hope this information helps. Please do not hesitate to contact us if you need anything further.

Sign up