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I would like to know if this kit can be used with paraffin-embedded (IHC-P) or frozen (IHC-Fr) tissues.
Asked on Jan 06 2011
Thank you for contacting us. This kit can only be used with fresh cells and tissue samples. Any process that leads to membrane damage like fixation and/or freeze-thaw cycles makes the sample incompatible with this kit. So unfortunately it cannot be used with IHC-P. However, we do have quite a few anti-Annexin V antibodies that work well in IHC-P. such as ab14196. https://www.abcam.com/Annexin-V-antibody-ab14196.html I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Jan 06 2011