Question (21979) | Annexin V-Cy3 Apoptosis Staining / Detection Kit (ab14142)

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Question

I would like to know if this kit can be used with paraffin-embedded (IHC-P) or frozen (IHC-Fr) tissues.

Answer

Thank you for contacting us. This kit can only be used with fresh cells and tissue samples. Any process that leads to membrane damage like fixation and/or freeze-thaw cycles makes the sample incompatible with this kit. So unfortunately it cannot be used with IHC-P. However, we do have quite a few anti-Annexin V antibodies that work well in IHC-P. such as ab14196. https://www.abcam.com/Annexin-V-antibody-ab14196.html I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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