Annexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX (ab14144)

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Overview

  • Product name
    Annexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX
    See all Annexin V kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Direct
  • Assay time
    0h 10m
  • Product overview

    AAnnexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX (ab14144) includes Annexin V-Cy3, SYTOX green dye, and binding buffer. The SYTOX green dye is impermeant to live cells and apoptotic cells, but stains necrotic cells with intense green fluorescence by binding to cellular nucleic acids. After staining a cell population with annexin V-Cy3 and SYTOX Green dye in the provided binding buffer, apoptotic cells show red fluorescence, dead cells show green fluorescence and live cells show little or no fluorescence. These populations can easily be distinguished by Fluorescence microscopy using FITC and rhodamine filters or by flow cytometry using the FL1 channel (Ex. 488 nm/Em. 530 nm) for SYTOX Green dye and FL2 channel for Annexin V-Cy3 (Ex. 543 nm/Em. 570 nm).
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    The assay is based on the observation that soon after initiating apoptosis, cells translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily be detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need of fixation.

  • Tested applications
    Suitable for: Flow Cyt, FMmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab14144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent dilution.
FM Use at an assay dependent concentration.

References

This product has been referenced in:
  • Alshatwi AA  et al. The apoptotic effect of hesperetin on human cervical cancer cells is mediated through cell cycle arrest, death receptor, and mitochondrial pathways. Fundam Clin Pharmacol N/A:N/A (2012). Read more (PubMed: 22913657) »
  • Ramesh E & Alshatwi AA Naringin induces death receptor and mitochondria-mediated apoptosis in human cervical cancer (SiHa) cells. Food Chem Toxicol 51C:97-105 (2012). Read more (PubMed: 22847135) »

See all 4 Publications for this product

Publishing research using ab14144? Please let us know so that we can cite the reference in this datasheet.

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Can this product be used with fixed cells?

Thank you for your enquiry. The Annexin V-Cy3 needs to be applied on live cells. If you choose to fix the cells after that, it will be fine. However, the Annexin V-cy3 will not bind to fixed cells.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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