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    annexin-v-cy3-apoptosis-staining--detection-kit-with-sytox-ab14144.pdf

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Cell Biology Apoptosis Annexin V Reagents
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Annexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX (ab14144)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (1)References (5)

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Key features and details

  • Assay type: Direct
  • Platform: Flow cytometer, Fluorescence microscope
  • Assay time: 10 min
  • Sample type: Adherent cells, Suspension cells

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Overview

  • Product name

    Annexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX
    See all Annexin V/ANXA5 kits
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Direct
  • Assay time

    0h 10m
  • Product overview

    Annexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX (ab14144) includes Annexin V-Cy3, SYTOX green dye, and binding buffer. It can be used in a 10 min, one-step staining procedure to detect apoptosis and necrosis. Analysis is by flow cytometry or fluorescence microscopy.


    The SYTOX green dye is impermeant to live cells and apoptotic cells, but stains necrotic cells with intense green fluorescence by binding to cellular nucleic acids.


    After staining a cell population with annexin V-Cy3 and SYTOX Green dye in binding buffer, apoptotic cells show red fluorescence, dead cells show green fluorescence and live cells show little or no fluorescence.


    These populations can easily be distinguished by fluorescence microscopy using FITC and rhodamine filters or by flow cytometry using the FL1 channel (Ex. 488 nm/Em. 530 nm) for SYTOX Green dye and FL2 channel for Annexin V-Cy3 (Ex. 543 nm/Em. 570 nm).


    The Annexin V-Cy3 reagent contained in the kit is also available as Annexin V-Cy3 reagent ab14082.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K202 Annexin V-Cy3 Apoptosis Kit Plus. K202-100 is the same size as the 100 test size of ab14144.

    The Annexin V apoptosis assay is based on the observation that soon after initiating apoptosis, cells translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily be detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. 

    For more apoptosis assays, review the full set of Annexin V assays, or the apoptosis assay and apoptosis marker guide.

  • Platform

    Flow cytometer, Fluorescence microscope

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 100 tests
    Annexin V-Cy3 1 x 500µl
    Binding Buffer 1 x 50ml
    SYTOX Green Dye 1 x 100µl
  • Research areas

    • Cell Biology
    • Apoptosis
    • Annexin V Reagents
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Detection kits
    • Annexin V
    • Cardiovascular
    • Atherosclerosis
    • Apoptosis
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Apoptosis marker and proteins ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • Apoptosis Kits
    • Annexin V Assays
    • Annexin V-Cy3
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Annexin V
    • Annexin V Detection Kits
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Annexin V
    • Annexin V Reagents
  • Function

    This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • Involvement in disease

    Pregnancy loss, recurrent, 3
  • Sequence similarities

    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain

    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications

    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Target information above from: UniProt accession P08758 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Anchorin CII
    • Annexin 5
    • Annexin A5
    • Annexin V
    • Annexin-5
    • Annexin5
    • AnnexinA5
    • AnnexinV
    • ANX 5
    • ANX A5
    • ANX5
    • ANXA5
    • ANXA5_HUMAN
    • Calphobindin I
    • CBP-I
    • Endonexin II
    • ENX 2
    • ENX2
    • Lipocortin V
    • PAP I
    • PAP-I
    • Placental anticoagulant protein 4
    • Placental anticoagulant protein I
    • PLACENTAL PROTEIN 4
    • PP 4
    • Pp4
    • RPRGL3
    • Thromboplastin inhibitor
    • VAC-alpha
    • Vascular anticoagulant-alpha
    see all

Associated products

  • Related Products

    • FCCP, mitochondrial oxidative phosphorylation uncoupler (ab120081)
    • Cycloheximide, Protein synthesis inhibitor (ab120093)
    • SB431542, ALK inhibitor (ab120163)
    • Z-VAD(OH)-FMK, Irreversible general caspase inhibitor (ab120382)
    • Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor (ab120487)
    • Doxorubicin hydrochloride, Topoisomerase II inhibitor (ab120629)
    • Dorsomorphin (Compound C), AMP-kinase inhibitor (ab120843)
    • Annexin V/ANXA5-FITC Apoptosis Detection Reagent (500X) (ab14082)
    • Propidium Iodide (ab14083)
    • 10X Annexin V/ANXA5 Binding Buffer (ab14084)
    • Annexin V-FITC Apoptosis Staining / Detection Kit (ab14085)
    • Annexin V-Cy3 Apoptosis Staining / Detection Kit (ab14142)
    • Calcein AM, fluorescent dye for cell viability (ab141420)
    • Annexin V/ANXA5-Cy3 Apoptosis Staining / Detection Reagent (ab14143)
    • Annexin V/ANXA5-Cy5 Apoptosis Staining / Detection Reagent (ab14147)
    • Annexin V-Cy5 Apoptosis Staining / Detection Kit (ab14150)
    • Annexin V-EGFP Apoptosis Staining / Detection Reagent (ab14152)
    • Annexin V-EGFP Apoptosis Staining / Detection Kit (ab14153)
    • Annexin V/ANXA5-PE Apoptosis Staining / Detection Reagent (ab14154)
    • Annexin V-PE Apoptosis Staining / Detection Kit (ab14155)
    • Annexin V-PE-Cy5 Apoptosis Staining / Detection Kit (ab14159)
    • Annexin V/ANXA5-Biotin Apoptosis Staining / Detection Reagent (ab14165)

Protocols

  • Annexin V-Cy3 Apoptosis Detection Kit with SYTOX
  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (5)

Publishing research using ab14144? Please let us know so that we can cite the reference in this datasheet.

ab14144 has been referenced in 5 publications.

  • Du Z  et al. Meningioma transcription factors link cell lineage with systemic metabolic cues. Neuro Oncol 20:1331-1343 (2018). PubMed: 29660031
  • Alshatwi AA  et al. The apoptotic effect of hesperetin on human cervical cancer cells is mediated through cell cycle arrest, death receptor, and mitochondrial pathways. Fundam Clin Pharmacol N/A:N/A (2012). PubMed: 22913657
  • Ramesh E & Alshatwi AA Naringin induces death receptor and mitochondria-mediated apoptosis in human cervical cancer (SiHa) cells. Food Chem Toxicol 51C:97-105 (2012). PubMed: 22847135
  • Pliss A  et al. From the Cover: Biophotonic probing of macromolecular transformations during apoptosis. Proc Natl Acad Sci U S A 107:12771-6 (2010). PubMed: 20615987
  • Honma K  et al. TNFAIP3/A20 functions as a novel tumor suppressor gene in several subtypes of non-Hodgkin lymphomas. Blood 114:2467-75 (2009). Human . PubMed: 19608751

Customer reviews and Q&As

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Question

Can this product be used with fixed cells?

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Abcam community

Verified customer

Asked on Nov 24 2008

Answer

Thank you for your enquiry. The Annexin V-Cy3 needs to be applied on live cells. If you choose to fix the cells after that, it will be fine. However, the Annexin V-cy3 will not bind to fixed cells.

Read More

Abcam Scientific Support

Answered on Nov 24 2008

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