Annexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX (ab14144)

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Overview

  • Product name

    Annexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX
    See all Annexin V/ANXA5 kits

  • Sample type

    Adherent cells, Suspension cells

  • Assay type

    Direct

  • Assay time

    0h 10m

  • Product overview

    Annexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX (ab14144) includes Annexin V-Cy3, SYTOX green dye, and binding buffer. It can be used in a 10 min, one-step staining procedure to detect apoptosis and necrosis. Analysis is by flow cytometry or fluorescence microscopy.


    The SYTOX green dye is impermeant to live cells and apoptotic cells, but stains necrotic cells with intense green fluorescence by binding to cellular nucleic acids.


    After staining a cell population with annexin V-Cy3 and SYTOX Green dye in binding buffer, apoptotic cells show red fluorescence, dead cells show green fluorescence and live cells show little or no fluorescence.


    These populations can easily be distinguished by fluorescence microscopy using FITC and rhodamine filters or by flow cytometry using the FL1 channel (Ex. 488 nm/Em. 530 nm) for SYTOX Green dye and FL2 channel for Annexin V-Cy3 (Ex. 543 nm/Em. 570 nm).


    The Annexin V-Cy3 reagent contained in the kit is also available as Annexin V-Cy3 reagent ab14082.

  • Notes

    The Annexin V apoptosis assay is based on the observation that soon after initiating apoptosis, cells translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily be detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. 

    For more apoptosis assays, review the full set of Annexin V assays, or the apoptosis assay and apoptosis marker guide.

  • Platform

    Flow cytometer, Fluorescence microscope

Properties

References

Publishing research using ab14144? Please let us know so that we can cite the reference in this datasheet.

ab14144 has been referenced in 5 publications.

  • Du Z  et al. Meningioma transcription factors link cell lineage with systemic metabolic cues. Neuro Oncol 20:1331-1343 (2018). PubMed: 29660031
  • Alshatwi AA  et al. The apoptotic effect of hesperetin on human cervical cancer cells is mediated through cell cycle arrest, death receptor, and mitochondrial pathways. Fundam Clin Pharmacol N/A:N/A (2012). PubMed: 22913657
  • Ramesh E & Alshatwi AA Naringin induces death receptor and mitochondria-mediated apoptosis in human cervical cancer (SiHa) cells. Food Chem Toxicol 51C:97-105 (2012). PubMed: 22847135
  • Pliss A  et al. From the Cover: Biophotonic probing of macromolecular transformations during apoptosis. Proc Natl Acad Sci U S A 107:12771-6 (2010). PubMed: 20615987
  • Honma K  et al. TNFAIP3/A20 functions as a novel tumor suppressor gene in several subtypes of non-Hodgkin lymphomas. Blood 114:2467-75 (2009). Human . PubMed: 19608751

Customer reviews and Q&As

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Question

Can this product be used with fixed cells?

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Answer

Thank you for your enquiry. The Annexin V-Cy3 needs to be applied on live cells. If you choose to fix the cells after that, it will be fine. However, the Annexin V-cy3 will not bind to fixed cells.

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