Overview

  • Product name
    Annexin V-Cy3 Apoptosis Staining / Detection Reagent
    See all Annexin V reagents
  • Tested applications
    Suitable for: FM, Flow Cytmore details
  • General notes

    Annexin V-Cy3 Apoptosis Detection Reagent ab14143 can be used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.

    This Annexin V-Cy3 reagent can be used with Annexin V binding buffer ab14084 (see protocol section for instructions). The Annexin V-Cy3 reagent is also available as part of Annexin V-Cy3 apoptosis kit ab14142 and Annexin V-Cy3 with SYTOX apoptosis kit ab14144.

    For more apoptosis assays, review the full set of Annexin V assays, or the apoptosis assay and apoptosis marker guide.

Properties

Applications

Our Abpromise guarantee covers the use of ab14143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.

References

This product has been referenced in:
  • Schuhmann D  et al. Interfering with interferon-? signalling in intestinal epithelial cells: selective inhibition of apoptosis-maintained secretion of anti-inflammatory interleukin-18 binding protein. Clin Exp Immunol 163:65-76 (2011). Read more (PubMed: 21078084) »
  • Thuwanut P  et al. The effects of antioxidants on semen traits and in vitro fertilizing ability of sperm from the flat-headed cat (Prionailurus planiceps). Theriogenology 76:115-25 (2011). Read more (PubMed: 21396691) »
See all 4 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your enquiry. Please find below the answers to your questions. 1) The source of the annexin is human recombinant protein. 2) This product is from an originator outside of Abcam. Unfortunately, due to company policies and supplier agreements, we are not able to disclose the supplier information. 3) The protein:dye ratio is, 1 PROTEIN:4 DYE 4) The dye binds randomly to the lysine amino acid. 5) We use the annexin V-Cye3 on apoptotic Jurket cell and watch for red color expression around the cells on a flow cytometer. If there is anything else that I can help you with, please do not hesitate to contact me.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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