Overview

  • Product name
    Annexin V-Cy5 Apoptosis Staining / Detection Kit
    See all Annexin V kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Direct
  • Assay time
    0h 10m
  • Product overview

    Annexin V-Cy5 Apoptosis Staining / Detection Kit ab14150 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.


    The Annexin V-Cy5 reagent contained in the kit is also available as Annexin V-Cy5 reagent ab14147.

  • Notes

    Soon after initiating apoptosis, cells translocate membrane phosphatidylserine molecules from the inner face of the plasma membrane to the cell surface. Phosphatidylserine on the cell surface is detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for phosphatidylserine.

    For more apoptosis assays, review the full set of Annexin V assays, or the apoptosis assay and apoptosis marker guide.

  • Platform
    Flow cytometer, Fluorescence microscope

Properties

Images

  • Vero cells were co-transfected with different Rep-constructs together with a plasmid expressing eGFP to identify successful transfection. After three days, cells were stained using Annexin V-Cy5 Apoptosis staining/detection kit (ab14150) and analyzed by flow cytometry with filters for eGFP and Cy5.  

References

This product has been referenced in:
  • Mayorga ME  et al. Role of SDF-1:CXCR4 in Impaired Post-Myocardial Infarction Cardiac Repair in Diabetes. Stem Cells Transl Med 7:115-124 (2018). Functional Studies ; Mouse . Read more (PubMed: 29119710) »
  • Lin Z  et al. Inhibition of the CyclinD1 promoter in response to sonic hedgehog signaling pathway transduction is mediated by Gli1. Exp Ther Med 13:307-314 (2017). Read more (PubMed: 28123507) »
See all 9 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your enquiry. I can confirm the concentration of annexin V in the ab14150 kit is 30 ug/ml. I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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