Overview

  • Product name

    Annexin V- DY-634 PI Apoptosis Staining / Detection Kit
    See all Annexin V/ANXA5 kits
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based (quantitative)
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Annexin V- DY-634 PI Apoptosis Staining / Detection Kit (ab214484) contains Annexin V labeled with DY-634, which allows the identification and quantitation of apoptotic cells on a single-cell basis by flow cytometry. Simultaneous staining of cells with Annexin V – DY634 (red fluorescence) and the non-vital dye propidium iodide (PI) (orange fluorescence) allows the discrimination of intact cells (AnnexinV-DY-634 negative, PI Staining Solution negative), early apoptotic (Annexin V-DY-634 positive, PI Staining Solution negative) and late apoptotic or necrotic cells (Annexin V-DY-634 positive, PI Staining Solution positive).


    DY-634 can be used as direct replacement for APC.


    For more apoptosis assays, review the full set of Annexin V assays, or the apoptosis assay and apoptosis marker guide.

  • Notes

    Apoptosis is a regulated process of cell death that occurs during embryonic development as well as maintenance of tissue homeostasis. Inappropriately regulated apoptosis is implicated in different disease states, such as neurodegeneration disease and cancer. The apoptosis program is characterized by morphologic features, including loss of plasma membrane asymmetry and attachment, condensation off the cytoplasm and nucleus, and compaction and fragmentation of the nuclear chromatin. Exposure of phosphatidylserine (PS) on the external surface of the cell membrane has been reported to occur in the early phases of apoptotic cell death, during which the cell membrane remains intact. In leukocyte apoptosis, PS on the outer surface of the cell marks the cell for recognition and phagocytosis by macrophages. The human vascular anticoagulant, annexin V, is a 35-36 kDa Ca2+ dependent phospholipid binding protein that has a high affinity for PS, and shows minimal binding to phosphatidylcholine and sphingomyelin. Changes in PS asymmetry, which can be analyzed by measuring annexin V binding to the cell membrane, are generally observed before morphological changes associated with apoptosis occurred and before membrane integrity is lost.

  • Platform

    Flow cytometer

Properties

Images

  • Untreated Jurakt cells were stained with Annexin V-DY-634/PI Apoptosis Detection Kit (ab214484), following assay protocol.

  • Jurakt cells treated with 6 μM camptothecin for four hours were stained with Annexin V-DY-634/PI Apoptosis Detection Kit (ab214484), following assay protocol. Gating showing the different populations of live, dead and apoptotic cells are shown in the figure.

Protocols

References

ab214484 has not yet been referenced specifically in any publications.

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