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    annexin-v-dy-634-pi-apoptosis-staining--detection-kit-ab214484.pdf

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Cell Biology Apoptosis Annexin V Reagents
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Annexin V- DY-634 PI Apoptosis Staining / Detection Kit (ab214484)

  • Datasheet
  • SDS
  • Protocol Booklet
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Annexin V-DY-634/PI Apoptosis Detection Kit (ab214484)
  • Annexin V-DY-634/PI Apoptosis Detection Kit (ab214484)

Key features and details

  • Assay type: Cell-based (quantitative)
  • Detection method: Fluorescent
  • Platform: Flow cytometer
  • Sample type: Adherent cells, Suspension cells

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Overview

  • Product name

    Annexin V- DY-634 PI Apoptosis Staining / Detection Kit
    See all Annexin V/ANXA5 kits
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based (quantitative)
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Annexin V- DY-634 PI Apoptosis Staining / Detection Kit (ab214484) contains Annexin V labeled with DY-634, which allows the identification and quantitation of apoptotic cells on a single-cell basis by flow cytometry. Simultaneous staining of cells with Annexin V – DY634 (red fluorescence) and the non-vital dye propidium iodide (PI) (orange fluorescence) allows the discrimination of intact cells (AnnexinV-DY-634 negative, PI Staining Solution negative), early apoptotic (Annexin V-DY-634 positive, PI Staining Solution negative) and late apoptotic or necrotic cells (Annexin V-DY-634 positive, PI Staining Solution positive).


    DY-634 can be used as direct replacement for APC.


    For more apoptosis assays, review the full set of Annexin V assays, or the apoptosis assay and apoptosis marker guide.

  • Notes

    Apoptosis is a regulated process of cell death that occurs during embryonic development as well as maintenance of tissue homeostasis. Inappropriately regulated apoptosis is implicated in different disease states, such as neurodegeneration disease and cancer. The apoptosis program is characterized by morphologic features, including loss of plasma membrane asymmetry and attachment, condensation off the cytoplasm and nucleus, and compaction and fragmentation of the nuclear chromatin. Exposure of phosphatidylserine (PS) on the external surface of the cell membrane has been reported to occur in the early phases of apoptotic cell death, during which the cell membrane remains intact. In leukocyte apoptosis, PS on the outer surface of the cell marks the cell for recognition and phagocytosis by macrophages. The human vascular anticoagulant, annexin V, is a 35-36 kDa Ca2+ dependent phospholipid binding protein that has a high affinity for PS, and shows minimal binding to phosphatidylcholine and sphingomyelin. Changes in PS asymmetry, which can be analyzed by measuring annexin V binding to the cell membrane, are generally observed before morphological changes associated with apoptosis occurred and before membrane integrity is lost.

  • Platform

    Flow cytometer

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 100 tests
    Annexin V DY-634 Conjugate (in PBS, pH 7.4) 1 x 500µl
    Propidium Iodide Staining Solution (in PBS, pH 7.4) 1 x 500µl
    10X Binding Buffer Concentrate 1 x 50ml
  • Research areas

    • Cell Biology
    • Apoptosis
    • Annexin V Reagents
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Detection kits
    • Annexin V
    • Cardiovascular
    • Atherosclerosis
    • Apoptosis
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Apoptosis marker and proteins ELISA kits
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Annexin V
    • Annexin V Detection Kits
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Annexin V
    • Annexin V Reagents
  • Function

    This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • Involvement in disease

    Pregnancy loss, recurrent, 3
  • Sequence similarities

    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain

    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications

    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Target information above from: UniProt accession P08758 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Anchorin CII
    • Annexin 5
    • Annexin A5
    • Annexin V
    • Annexin-5
    • Annexin5
    • AnnexinA5
    • AnnexinV
    • ANX 5
    • ANX A5
    • ANX5
    • ANXA5
    • ANXA5_HUMAN
    • Calphobindin I
    • CBP-I
    • Endonexin II
    • ENX 2
    • ENX2
    • Lipocortin V
    • PAP I
    • PAP-I
    • Placental anticoagulant protein 4
    • Placental anticoagulant protein I
    • PLACENTAL PROTEIN 4
    • PP 4
    • Pp4
    • RPRGL3
    • Thromboplastin inhibitor
    • VAC-alpha
    • Vascular anticoagulant-alpha
    see all

Associated products

  • Related Products

    • FCCP, mitochondrial oxidative phosphorylation uncoupler (ab120081)
    • Cycloheximide, Protein synthesis inhibitor (ab120093)
    • SB431542, ALK inhibitor (ab120163)
    • Z-VAD(OH)-FMK, Irreversible general caspase inhibitor (ab120382)
    • Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor (ab120487)
    • Doxorubicin hydrochloride, Topoisomerase II inhibitor (ab120629)
    • Dorsomorphin (Compound C), AMP-kinase inhibitor (ab120843)
    • Annexin V/ANXA5-FITC Apoptosis Detection Reagent (500X) (ab14082)
    • Propidium Iodide (ab14083)
    • 10X Annexin V/ANXA5 Binding Buffer (ab14084)
    • Annexin V-Cy3 Apoptosis Staining / Detection Kit (ab14142)
    • Calcein AM, fluorescent dye for cell viability (ab141420)
    • Annexin V/ANXA5-Cy3 Apoptosis Staining / Detection Reagent (ab14143)
    • Annexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX (ab14144)
    • Annexin V/ANXA5-Cy5 Apoptosis Staining / Detection Reagent (ab14147)
    • Annexin V-Cy5 Apoptosis Staining / Detection Kit (ab14150)
    • Annexin V-EGFP Apoptosis Staining / Detection Reagent (ab14152)
    • Annexin V-EGFP Apoptosis Staining / Detection Kit (ab14153)
    • Annexin V/ANXA5-PE Apoptosis Staining / Detection Reagent (ab14154)
    • Annexin V-PE Apoptosis Staining / Detection Kit (ab14155)
    • Annexin V-PE-Cy5 Apoptosis Staining / Detection Kit (ab14159)
    • Annexin V/ANXA5-Biotin Apoptosis Staining / Detection Reagent (ab14165)
    • Annexin V-CF Blue PI Apoptosis Staining / Detection Kit (ab214485)
    • Annexin V-CF Blue 7-AAD Apoptosis Staining / Detection Kit (ab214663)

Images

  • Annexin V-DY-634/PI Apoptosis Detection Kit (ab214484)
    Annexin V-DY-634/PI Apoptosis Detection Kit (ab214484)

    Untreated Jurkat cells were stained with Annexin V-DY-634/PI Apoptosis Detection Kit (ab214484), following assay protocol.

  • Annexin V-DY-634/PI Apoptosis Detection Kit (ab214484)
    Annexin V-DY-634/PI Apoptosis Detection Kit (ab214484)

    Jurkat cells treated with 6 μM camptothecin for four hours were stained with Annexin V-DY-634/PI Apoptosis Detection Kit (ab214484), following assay protocol. Gating showing the different populations of live, dead and apoptotic cells are shown in the figure.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab214484? Please let us know so that we can cite the reference in this datasheet.

ab214484 has been referenced in 1 publication.

  • Po WW  et al. Fluoxetine Simultaneously Induces Both Apoptosis and Autophagy in Human Gastric Adenocarcinoma Cells. Biomol Ther (Seoul) 28:202-210 (2020). PubMed: 31522488

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