Overview

  • Product name
    Annexin V-EGFP Apoptosis Staining / Detection Kit
    See all Annexin V/ANXA5 kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Direct
  • Assay time
    0h 10m
  • Product overview

     


    Annexin V-EGFP Apoptosis Staining / Detection Kit ab14153 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy with a FITC filter.


    EGFP is brighter and more photo-stable than other fluorescent reagents. The kit can differentiate apoptosis vs necrosis when performing both annexin V-EGFP and PI staining.

  • Notes

    Soon after initiating apoptosis, cells translocate membrane phosphatidylserine molecules from the inner face of the plasma membrane to the cell surface. Phosphatidylserine on the cell surface is detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for phosphatidylserine.

    For more apoptosis assays, review the full set of Annexin V assays, or the apoptosis assay and apoptosis marker guide.

  • Platform
    Flow cytometer, Fluorescence microscope

Properties

Protocols

References

This product has been referenced in:
  • Jagannathan R  et al. AJUBA LIM Proteins Limit Hippo Activity in Proliferating Cells by Sequestering the Hippo Core Kinase Complex in the Cytosol. Mol Cell Biol 36:2526-42 (2016). Read more (PubMed: 27457617) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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