• Product name

    Annexin V-iFluor 488 Apoptosis Detection Kit
    See all Annexin V/ANXA5 kits
  • Detection method

  • Sample type

    Adherent cells, Suspension cells
  • Assay type

  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Annexin V-iFluor 488 Apoptosis Detection Kit (ab219916) contains Annexin V labeled with our proprietary green fluorescent dye iFluor 488, which allows the identification and quantitation of apoptotic cells on a single-cell basis by flow cytometry. Simultaneous staining of cells with Annexin V-iFluor488 (green fluorescence) and the non-vital dye propidium iodide (PI) (orange fluorescence) allows the discrimination of intact cells (Annexin V-iFluor 488 negative, PI Staining Solution negative), early apoptotic (Annexin V-iFluor 488 positive, PI Staining Solution negative) and late apoptotic or necrotic cells (Annexin V-iFluor 488, PI Staining Solution positive).

    The iFluor 488 dye (Ex/Em = 490/525 nm) has spectral properties almost identical to those of FITC or Alexa Fluor® 488, making it convenient to be used with the common fluorescence instruments equipped with the light sources and filters for FITC, the most common fluorophore.

  • Notes

    Apoptosis is a regulated process of cell death that occurs during embryonic development as well as maintenance of tissue homeostasis. Inappropriately regulated apoptosis is implicated in different disease states, such as neurodegeneration disease and cancer. The apoptosis program is characterized by morphologic features, including loss of plasma membrane asymmetry and attachment, condensation off the cytoplasm and nucleus, and compaction and fragmentation of the nuclear chromatin. Exposure of phosphatidylserine (PS) on the external surface of the cell membrane has been reported to occur in the early phases of apoptotic cell death, during which the cell membrane remains intact. In leukocyte apoptosis, PS on the outer surface of the cell marks the cell for recognition and phagocytosis by macrophages. The human vascular anticoagulant, annexin V, is a 35-36 kDa Ca2+ dependent phospholipid binding protein that has a high affinity for PS, and shows minimal binding to phosphatidylcholine and sphingomyelin. Changes in PS asymmetry, which can be analyzed by measuring annexin V binding to the cell membrane, are generally observed before morphological changes associated with apoptosis occurred and before membrane integrity is lost.


    Alexa Fluor® 488 is the trademark of Invitrogen.

  • Platform

    Flow cytometer, Fluorescence microscope



  • Annexin V-iFluor 488 Apoptosis Detection Kit (ab219916). Detection of phosphatidylserine (PS) exposure in Jurkat cells. Jurkat cells were left untreated (blue) or treated with 20 μM camptothecin (red) in a 37°C, 5% CO2 incubator for 4-5 hours. Cells were then incubated with Annexin V-iFluor reagent and PI for 30 minutes. The fluorescence intensitiy of Annexin V-iFluor 488 was measured with a FACSCalibur (BD Systems) flow cytometer using the FL1channel.

    In live non-apoptotic cells, Annexin V-iFluor 488 conjugate detects innate apoptosis in non-induced cells, which is typically 2-6% of all cells. In apoptotic cells Annexin V-iFluor 488 conjugate binds to phosphatidylserine, which is located on the outer leaflet of the cell membrane, resulted in increased staining intensity.



ab219916 has not yet been referenced specifically in any publications.

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