For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    annexin-v-ifluor-647-apoptosis-detection-kit-ab219919.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Apoptosis Annexin V Reagents
Share by email

Annexin V-iFluor 647 Apoptosis Detection Kit (ab219919)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

The detection of binding activity of Annexin V-iFluor 647 and phosphatidylserine in Jurkat cells.

    Key features and details

    • Assay type: Quantitative
    • Detection method: Fluorescent
    • Platform: Flow cytometer, Fluorescence microscope
    • Sample type: Adherent cells, Suspension cells

    You may also be interested in

    Assay
    Product image
    Propidium Iodide (ab14083)
    Biochemical
    SAHA (Vorinostat), HDAC inhibitor (ab144480)
    Primary
    Product image
    Anti-GDF8 / Myostatin antibody (ab203076)

    View more associated products

    Overview

    • Product name

      Annexin V-iFluor 647 Apoptosis Detection Kit
      See all Annexin V/ANXA5 kits
    • Detection method

      Fluorescent
    • Sample type

      Adherent cells, Suspension cells
    • Assay type

      Quantitative
    • Species reactivity

      Reacts with: Mammals, Other species
    • Product overview

      Annexin V-iFluor 647 Apoptosis Detection Kit (ab219919) contains Annexin V labeled with our proprietary orange fluorescent dye iFluor 647, which allows the identification and quantification of apoptotic cells on a single-cell basis by flow cytometry or fluorescence microscopy.


      The iFluor 647 dye (Ex/Em = 650/680 nm) has spectral properties almost identical to those of Cy5® or Alexa Fluor® 647, making it convenient to be used for common fluorescence instruments equipped with the light sources and filters for Cy5® or Alexa Fluor® 647.

    • Notes

      Annexins are a family of calcium-dependent phospholipid-binding proteins. They are abundant in eukaryotic organisms belonging to a family of ubiquitous

      Apoptosis is a regulated process of cell death that occurs during embryonic development as well as maintenance of tissue homeostasis. Inappropriately regulated apoptosis is implicated in different disease states, such as neurodegeneration disease and cancer. The apoptosis program is characterized by morphologic features, including loss of plasma membrane asymmetry and attachment, condensation off the cytoplasm and nucleus, and compaction and fragmentation of the nuclear chromatin. Exposure of phosphatidylserine (PS) on the external surface of the cell membrane has been reported to occur in the early phases of apoptotic cell death, during which the cell membrane remains intact. In leukocyte apoptosis, PS on the outer surface of the cell marks the cell for recognition and phagocytosis by macrophages. The human vascular anticoagulant, annexin V, is a 35-36 kDa Ca2+ dependent phospholipid binding protein that has a high affinity for PS, and shows minimal binding to phosphatidylcholine and sphingomyelin. Changes in PS asymmetry, which can be analyzed by measuring annexin V binding to the cell membrane, are generally observed before morphological changes associated with apoptosis occurred and before membrane integrity is lost.

       

      Cy5® and Alexa Fluor® 647 are the trademarks of GE Healthcare and Invitrogen respectively.

    • Platform

      Flow cytometer, Fluorescence microscope

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 100 tests
      Annexin 647 (100X stock solution) 1 x 100µl
      Assay Buffer 1 x 50ml
    • Research areas

      • Cell Biology
      • Apoptosis
      • Annexin V Reagents
      • Cancer
      • Invasion/microenvironment
      • Apoptosis
      • Detection kits
      • Annexin V
      • Cardiovascular
      • Atherosclerosis
      • Apoptosis
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Apoptosis marker and proteins ELISA kits
      • Cancer
      • Cell Death
      • Apoptosis
      • Apoptosis Markers
      • Annexin V
      • Annexin V Detection Kits
      • Cancer
      • Cell Death
      • Apoptosis
      • Apoptosis Markers
      • Annexin V
      • Annexin V Reagents
    • Function

      This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
    • Involvement in disease

      Pregnancy loss, recurrent, 3
    • Sequence similarities

      Belongs to the annexin family.
      Contains 4 annexin repeats.
    • Domain

      The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
      A pair of annexin repeats may form one binding site for calcium and phospholipid.
    • Post-translational
      modifications

      S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
    • Target information above from: UniProt accession P08758 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Anchorin CII
      • Annexin 5
      • Annexin A5
      • Annexin V
      • Annexin-5
      • Annexin5
      • AnnexinA5
      • AnnexinV
      • ANX 5
      • ANX A5
      • ANX5
      • ANXA5
      • ANXA5_HUMAN
      • Calphobindin I
      • CBP-I
      • Endonexin II
      • ENX 2
      • ENX2
      • Lipocortin V
      • PAP I
      • PAP-I
      • Placental anticoagulant protein 4
      • Placental anticoagulant protein I
      • PLACENTAL PROTEIN 4
      • PP 4
      • Pp4
      • RPRGL3
      • Thromboplastin inhibitor
      • VAC-alpha
      • Vascular anticoagulant-alpha
      see all

    Associated products

      Images

      • The detection of binding activity of Annexin V-iFluor 647 and phosphatidylserine in Jurkat cells.
        The detection of binding activity of Annexin V-iFluor 647 and phosphatidylserine in Jurkat cells.

        Annexin V-iFluor 647 Apoptosis Detection Kit (ab219919). Detection of phosphatidylserine (PS) exposure in Jurkat cells. Jurkat cells were left untreated (blue) or treated with 20 μM camptothecin (red) in a 37°C, 5% CO2 incubator for 4-5 hours. Cells were then incubated with Annexin V-iFluor reagent and PI for 30 minutes. The fluorescence intensitiy of Annexin V-iFluor 647 was measured with a FACSCalibur (BD Systems) flow cytometer using the FL41channel.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab219919? Please let us know so that we can cite the reference in this datasheet.

      ab219919 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab219919.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.