Overview

  • Product name

    Annexin V-mFluor Violet 550 Apoptosis Detection Kit
    See all Annexin V/ANXA5 kits
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Quantitative
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Annexin V-mFluor Violet 550 Apoptosis Detection Kit (ab219922) contains Annexin V labeled with our proprietary orange fluorescent dye mFluor Violet 550, which allows the identification and quantitation of apoptotic cells on a single-cell basis by flow cytometry. Simultaneous staining of cells with Annexin V-mFluor Violet 550 (orange fluorescence) and the non-vital dye propidium iodide (PI) allows the discrimination of intact cells (Annexin V-mFluor Violet 550 negative, PI Staining Solution negative), early apoptotic (Annexin V-mFluor Violet 550 positive, PI Staining Solution negative) and late apoptotic or necrotic cells (Annexin V-mFluor Violet 550, PI Staining Solution positive).


    The mFluor Violet 550 (Ex/Em = 405/550 nm, excited with the violet laser at 405 nm) has spectral properties almost identical to those of Pacific Orange®, making it convenient to be used with a fluorescence instruments equipped with a violet laser.

  • Notes

    Apoptosis is a regulated process of cell death that occurs during embryonic development as well as maintenance of tissue homeostasis. Inappropriately regulated apoptosis is implicated in different disease states, such as neurodegeneration disease and cancer. The apoptosis program is characterized by morphologic features, including loss of plasma membrane asymmetry and attachment, condensation off the cytoplasm and nucleus, and compaction and fragmentation of the nuclear chromatin. Exposure of phosphatidylserine (PS) on the external surface of the cell membrane has been reported to occur in the early phases of apoptotic cell death, during which the cell membrane remains intact. In leukocyte apoptosis, PS on the outer surface of the cell marks the cell for recognition and phagocytosis by macrophages. The human vascular anticoagulant, annexin V, is a 35-36 kDa Ca2+ dependent phospholipid binding protein that has a high affinity for PS, and shows minimal binding to phosphatidylcholine and sphingomyelin. Changes in PS asymmetry, which can be analyzed by measuring annexin V binding to the cell membrane, are generally observed before morphological changes associated with apoptosis occurred and before membrane integrity is lost.

     

    Pacific Orange® is the trademark of Invitrogen.

  • Platform

    Flow cytometer

Properties

Images

  • Annexin V-mFluor Violet 550 Apoptosis Detection Kit (ab219922). Detection of phosphatidylserine (PS) exposure in Jurkat cells. Jurkat cells were left untreated (blue) or treated with 20 μM camptothecin (red) in a 37°C, 5% CO2 incubator for 4-5 hours. Cells were then incubated with Annexin V-mFluor Violet 550 reagent and PI for 30 minutes. The fluorescence intensitiy of Annexin V-mFluor Violet 550 was measured with a FACSCalibur (BD Systems) flow cytometer using the Violet channel.

    In live non-apoptotic cells, Annexin V-mFluor Violet 550 conjugate detects innate apoptosis in non-induced cells, which is typically 2-6% of all cells. In apoptotic cells Annexin V-mFluor Violet 550 conjugate binds to phosphatidylserine, which is located on the outer leaflet of the cell membrane, resulted in increased staining intensity.

Protocols

References

ab219922 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab219922.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up