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    annexin-v-pe-apoptosis-staining--detection-kit-ab14155.pdf

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Cell Biology Apoptosis Annexin V Reagents
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Annexin V-PE Apoptosis Staining / Detection Kit (ab14155)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (4)References (9)

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Functional assays: Annexin V-PE Apoptosis Detection Kit (ab14155)

    Key features and details

    • Assay type: Direct
    • Platform: Flow cytometer, Fluorescence microscope
    • Assay time: 10 min
    • Sample type: Adherent cells, Suspension cells

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    Overview

    • Product name

      Annexin V-PE Apoptosis Staining / Detection Kit
      See all Annexin V/ANXA5 kits
    • Sample type

      Adherent cells, Suspension cells
    • Assay type

      Direct
    • Assay time

      0h 10m
    • Product overview

      Annexin V-PE Apoptosis Staining / Detection Kit ab14155 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.


      The Annexin V-PE reagent contained in the kit is also available as Annexin V-PE reagent ab14154.

    • Notes

      This product is manufactured by BioVision, an Abcam company and was previously called K128 Annexin V-PE Apoptosis Detection Kit. K128-100 is the same size as the 100 test size of ab14155.

      Soon after initiating apoptosis, cells translocate membrane phosphatidylserine molecules from the inner face of the plasma membrane to the cell surface. Phosphatidylserine on the cell surface is detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for phosphatidylserine.

      For more apoptosis assays, review the full set of Annexin V assays, or the apoptosis assay and apoptosis marker guide.

    • Platform

      Flow cytometer, Fluorescence microscope

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 100 tests
      Annexin V-PE 1 x 500µl
      Binding Buffer 1 x 50ml
    • Research areas

      • Cell Biology
      • Apoptosis
      • Annexin V Reagents
      • Cancer
      • Invasion/microenvironment
      • Apoptosis
      • Detection kits
      • Annexin V
      • Cardiovascular
      • Atherosclerosis
      • Apoptosis
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Apoptosis marker and proteins ELISA kits
      • Kits/ Lysates/ Other
      • Kits
      • Apoptosis Kits
      • Annexin V Assays
      • Annexin V-PE
      • Cancer
      • Cell Death
      • Apoptosis
      • Apoptosis Markers
      • Annexin V
      • Annexin V Detection Kits
      • Cancer
      • Cell Death
      • Apoptosis
      • Apoptosis Markers
      • Annexin V
      • Annexin V Reagents
    • Function

      This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
    • Involvement in disease

      Pregnancy loss, recurrent, 3
    • Sequence similarities

      Belongs to the annexin family.
      Contains 4 annexin repeats.
    • Domain

      The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
      A pair of annexin repeats may form one binding site for calcium and phospholipid.
    • Post-translational
      modifications

      S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
    • Target information above from: UniProt accession P08758 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Anchorin CII
      • Annexin 5
      • Annexin A5
      • Annexin V
      • Annexin-5
      • Annexin5
      • AnnexinA5
      • AnnexinV
      • ANX 5
      • ANX A5
      • ANX5
      • ANXA5
      • ANXA5_HUMAN
      • Calphobindin I
      • CBP-I
      • Endonexin II
      • ENX 2
      • ENX2
      • Lipocortin V
      • PAP I
      • PAP-I
      • Placental anticoagulant protein 4
      • Placental anticoagulant protein I
      • PLACENTAL PROTEIN 4
      • PP 4
      • Pp4
      • RPRGL3
      • Thromboplastin inhibitor
      • VAC-alpha
      • Vascular anticoagulant-alpha
      see all

    Associated products

    • Related Products

      • FCCP, mitochondrial oxidative phosphorylation uncoupler (ab120081)
      • Cycloheximide, Protein synthesis inhibitor (ab120093)
      • SB431542, ALK inhibitor (ab120163)
      • Z-VAD(OH)-FMK, Irreversible general caspase inhibitor (ab120382)
      • Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor (ab120487)
      • Doxorubicin hydrochloride, Topoisomerase II inhibitor (ab120629)
      • Dorsomorphin (Compound C), AMP-kinase inhibitor (ab120843)
      • Annexin V/ANXA5-FITC Apoptosis Detection Reagent (500X) (ab14082)
      • Propidium Iodide (ab14083)
      • 10X Annexin V/ANXA5 Binding Buffer (ab14084)
      • Annexin V-FITC Apoptosis Staining / Detection Kit (ab14085)
      • Annexin V-Cy3 Apoptosis Staining / Detection Kit (ab14142)
      • Calcein AM, fluorescent dye for cell viability (ab141420)
      • Annexin V/ANXA5-Cy3 Apoptosis Staining / Detection Reagent (ab14143)
      • Annexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX (ab14144)
      • Annexin V/ANXA5-Cy5 Apoptosis Staining / Detection Reagent (ab14147)
      • Annexin V-Cy5 Apoptosis Staining / Detection Kit (ab14150)
      • Annexin V-EGFP Apoptosis Staining / Detection Reagent (ab14152)
      • Annexin V-EGFP Apoptosis Staining / Detection Kit (ab14153)
      • Annexin V/ANXA5-PE Apoptosis Staining / Detection Reagent (ab14154)
      • Annexin V-PE-Cy5 Apoptosis Staining / Detection Kit (ab14159)
      • Annexin V/ANXA5-Biotin Apoptosis Staining / Detection Reagent (ab14165)

    Images

    • Functional assays: Annexin V-PE Apoptosis Detection Kit (ab14155)
      Functional assays: Annexin V-PE Apoptosis Detection Kit (ab14155)

      Annexin V detected in 10e4 control Jurkat cells (black) or in cells treated with 50 μM H2O2 (Sigma) for 6 hours (red).

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (9)

    Publishing research using ab14155? Please let us know so that we can cite the reference in this datasheet.

    ab14155 has been referenced in 9 publications.

    • Brescia M  et al. High-Capacity Adenoviral Vectors Permit Robust and Versatile Testing of DMD Gene Repair Tools and Strategies in Human Cells. Cells 9:N/A (2020). PubMed: 32252479
    • Xiang J  et al. Melatonin-induced ApoE expression in mouse astrocytes protects endothelial cells from OGD-R induced injuries. Transl Psychiatry 10:181 (2020). PubMed: 32513932
    • Tao B  et al. CA10 and CA11 negatively regulate neuronal activity-dependent growth of gliomas. Mol Oncol 13:1018-1032 (2019). PubMed: 30636076
    • Li S  et al. HOXC10 promotes proliferation and invasion and induces immunosuppressive gene expression in glioma. FEBS J 285:2278-2291 (2018). PubMed: 29676849
    • Witt AE  et al. Identification of a cancer stem cell-specific function for the histone deacetylases, HDAC1 and HDAC7, in breast and ovarian cancer. Oncogene 36:1707-1720 (2017). PubMed: 27694895
    • Alegret JM  et al. Data on the circulating levels of endothelial microparticles are elevated in patients with bicuspid aortic valve and are related to aortic dilation. Data Brief 8:666-9 (2016). PubMed: 27453924
    • Afzali M  et al. Non-addictive opium alkaloids selectively induce apoptosis in cancer cells compared to normal cells. Daru 23:16 (2015). PubMed: 25890335
    • Lee KS  et al. nc886, a non-coding RNA of anti-proliferative role, is suppressed by CpG DNA methylation in human gastric cancer. Oncotarget 5:3944-55 (2014). PubMed: 25003254
    • Stolzing A  et al. Glucose-induced replicative senescence in mesenchymal stem cells. Rejuvenation Res 9:31-5 (2006). PubMed: 16608393

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Question

    I had a query about Annexin V –PE kit Ab14155. In the attached data sheet the components are listed as Annexin V-PE/Cy5 rather than Annexin-PE. What is annexin V conjugated to in this kit?

    Regards

    Read More

    Abcam community

    Verified customer

    Asked on Sep 24 2012

    Answer

    Thank you for your enquiry.

    I have investigated our records for ab14155. I can confirm that this kit contains Annexin V-PE.

    The datasheet has been updated with the correct information and I apologize for any confusion. (Please note it can take a day or two for updated information to refresh on the internet).

    The quality of our products, kitsand datasheets is very important to us, as is any feedback and data we receive. It is regrettable that the information we had on this particular antibody was not of the standard both we and our customers expect from our products.

    Should you have any further questions regarding this issue, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Sep 24 2012

    Question


    I have recently purchased yourAnnexin V-PE Apoptosis Detection Kit (ab14155) to assay T cell apoptosis. For the assay I need an isotype control, however the data sheet does not mention what type of antibody the Annexin-V antibody is (e.g. mouse IgG1 etc). Whom should I contact to find out this information?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 18 2012

    Answer

    Thanks for your enquiry.

    The reagent that comes with this kit is not an antibody. It is PE/Cy5-labeled Annexin V protein. Annexin V will bind specifically to the membrane phospholipid phosphatidylserine, which in apoptotic cells has translocated from the inner cell membrane tothe cell surface.

    I hope this is helpful. Please contact us again if you have any further questions.

    Read More

    Abcam Scientific Support

    Answered on Sep 18 2012

    Question

    Dear Sir, Thank you for your kind help. I have an inquiry about your product, ab14155; Annexin V-PE Apoptosis Detection Kit. One of our customers used it with home-made 1X Binding buffer and all of cells died. The formulation of hom-made buffer is as follows. 1X HEPES 10mM pH7.4 NaCl 140mM CaCl2 2.5mM Could you please let me know your advices. Is this formulation OK?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 04 2006

    Answer

    The source of the Annexin V-PE Apoptosis Detection Kit informed me that the formulation of the buffer is confidential and therefore I am unable to compare it with the customer's home made buffer, I'm very sorry. The customer can buy more of the buffer which is reference ab14084. https://www.abcam.com/10X-Annexin-VANXA5-Binding-Buffer-ab14084.html I hope this will help,

    Read More

    Abcam Scientific Support

    Answered on Jul 10 2006

    Question

    We have been using Annexin V-PE kit (ab14155) and run out of the binding buffer. Is the buffer in this kit sold separately? Does your product annexin V 10X binding buffer (ab140804) work with this kit? We appreciate your help if you could respond it ASAP. Our experiment has been set up and we are waiting for the buffer.

    Read More

    Abcam community

    Verified customer

    Asked on May 02 2006

    Answer

    Thank you for your enquiry. Yes, ab14084 is the replacement buffer product for this kit. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on May 02 2006

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