Annexin V-PE Apoptosis Staining / Detection Kit (ab14155)
- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnnexin V-PE Apoptosis Staining / Detection Kit
See all Annexin V kits -
Sample typeAdherent cells, Suspension cells
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Assay typeDirect
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Assay time0h 10m
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Product overview
Annexin V-PE Apoptosis Staining / Detection Kit (ab14155) is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need for fixation.
Visit our FAQs page for tips and troubleshooting. -
Tested applicationsSuitable for: FM, Flow Cytmore details
Properties
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Storage instructionsStore at +4°C. Please refer to protocols.
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Components 100 tests Annexin V-PE 1 x 500µl Binding Buffer 1 x 50ml -
Research areas
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FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
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Involvement in diseasePregnancy loss, recurrent, 3
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Sequence similaritiesBelongs to the annexin family.
Contains 4 annexin repeats. -
DomainThe [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
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Alternative names
- Anchorin CII
- Annexin 5
- Annexin A5
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Associated products
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- Annexin V-FITC Apoptosis Detection Reagent (500X) (ab14082)
- Propidium Iodide (ab14083)
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- Annexin V-Cy5 Apoptosis Staining / Detection Reagent (ab14147)
- Annexin V-Cy5 Apoptosis Staining / Detection Kit (ab14150)
- Annexin V-EGFP Apoptosis Staining / Detection Kit (ab14153)
- Annexin V-PE Apoptosis Staining / Detection Reagent (ab14154)
- Annexin V-PE-Cy5 Apoptosis Staining / Detection Kit (ab14159)
- Annexin V-Biotin Apoptosis Staining / Detection Reagent (ab14165)
- Annexin V-Biotin Apoptosis Staining / Detection Kit (ab14190)
- Annexin V Unlabeled Apoptosis Reagent (ab14193)
Applications
Our Abpromise guarantee covers the use of ab14155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| FM | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent dilution. |
Images
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Functional assays: Annexin V-PE Apoptosis Detection Kit (ab14155)Annexin V detected in 10e4 control Jurkat cells (black) or in cells treated with 50 μM H2O2 (Sigma) for 6 hours (red).
Protocols
References
This product has been referenced in:
- Alegret JM et al. Data on the circulating levels of endothelial microparticles are elevated in patients with bicuspid aortic valve and are related to aortic dilation. Data Brief 8:666-9 (2016). Read more (PubMed: 27453924) »
- Stolzing A et al. Glucose-induced replicative senescence in mesenchymal stem cells. Rejuvenation Res 9:31-5 (2006). Read more (PubMed: 16608393) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"