Annexin V-PE Apoptosis Staining / Detection Kit (ab14155)

Submit a review Q&A (4)References (3)

Overview

  • Product name
    Annexin V-PE Apoptosis Staining / Detection Kit
    See all Annexin V kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Direct
  • Assay time
    0h 10m
  • Product overview

    Annexin V-PE Apoptosis Staining / Detection Kit (ab14155) is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need for fixation.
    Visit our FAQs page for tips and troubleshooting.

  • Tested applications
    Suitable for: FM, Flow Cytmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab14155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.

Images

  • Functional assays: Annexin V-PE Apoptosis Detection Kit (ab14155)
    Functional assays: Annexin V-PE Apoptosis Detection Kit (ab14155)

    Annexin V detected in 10e4 control Jurkat cells (black) or in cells treated with 50 μM H2O2 (Sigma) for 6 hours (red).

Protocols

References

This product has been referenced in:
  • Li S  et al. HOXC10 promotes proliferation and invasion and induces immunosuppressive gene expression in glioma. FEBS J 285:2278-2291 (2018). Read more (PubMed: 29676849) »
  • Alegret JM  et al. Data on the circulating levels of endothelial microparticles are elevated in patients with bicuspid aortic valve and are related to aortic dilation. Data Brief 8:666-9 (2016). Read more (PubMed: 27453924) »
See all 3 Publications for this product

Publishing research using ab14155? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Question

I had a query about Annexin V –PE kit Ab14155. In the attached data sheet the components are listed as Annexin V-PE/Cy5 rather than Annexin-PE. What is annexin V conjugated to in this kit?

Regards

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Answer

Thank you for your enquiry.

I have investigated our records for ab14155. I can confirm that this kit contains Annexin V-PE.

The datasheet has been updated with the correct information and I apologize for any confusion. (Please note it can take a day or two for updated information to refresh on the internet).

The quality of our products, kitsand datasheets is very important to us, as is any feedback and data we receive. It is regrettable that the information we had on this particular antibody was not of the standard both we and our customers expect from our products.

Should you have any further questions regarding this issue, please do not hesitate to contact me.

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Question


I have recently purchased yourAnnexin V-PE Apoptosis Detection Kit (ab14155) to assay T cell apoptosis. For the assay I need an isotype control, however the data sheet does not mention what type of antibody the Annexin-V antibody is (e.g. mouse IgG1 etc). Whom should I contact to find out this information?

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Answer

Thanks for your enquiry.

The reagent that comes with this kit is not an antibody. It is PE/Cy5-labeled Annexin V protein. Annexin V will bind specifically to the membrane phospholipid phosphatidylserine, which in apoptotic cells has translocated from the inner cell membrane tothe cell surface.

I hope this is helpful. Please contact us again if you have any further questions.

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Question

Dear Sir, Thank you for your kind help. I have an inquiry about your product, ab14155; Annexin V-PE Apoptosis Detection Kit. One of our customers used it with home-made 1X Binding buffer and all of cells died. The formulation of hom-made buffer is as follows. 1X HEPES 10mM pH7.4 NaCl 140mM CaCl2 2.5mM Could you please let me know your advices. Is this formulation OK?

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Answer

The source of the Annexin V-PE Apoptosis Detection Kit informed me that the formulation of the buffer is confidential and therefore I am unable to compare it with the customer's home made buffer, I'm very sorry. The customer can buy more of the buffer which is reference ab14084. https://www.abcam.com/index.html?datasheet=14084 I hope this will help,

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Question

We have been using Annexin V-PE kit (ab14155) and run out of the binding buffer. Is the buffer in this kit sold separately? Does your product annexin V 10X binding buffer (ab140804) work with this kit? We appreciate your help if you could respond it ASAP. Our experiment has been set up and we are waiting for the buffer.

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Answer

Thank you for your enquiry. Yes, ab14084 is the replacement buffer product for this kit. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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