Annexin V-PE Apoptosis Staining / Detection Kit (ab14155)
Key features and details
- Assay type: Direct
- Platform: Flow cytometer, Fluorescence microscope
- Assay time: 10 min
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Annexin V-PE Apoptosis Staining / Detection Kit
See all Annexin V/ANXA5 kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Direct -
Assay time
0h 10m -
Product overview
Annexin V-PE Apoptosis Staining / Detection Kit ab14155 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.
The Annexin V-PE reagent contained in the kit is also available as Annexin V-PE reagent ab14154.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K128 Annexin V-PE Apoptosis Detection Kit. K128-100 is the same size as the 100 test size of ab14155.
Soon after initiating apoptosis, cells translocate membrane phosphatidylserine molecules from the inner face of the plasma membrane to the cell surface. Phosphatidylserine on the cell surface is detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for phosphatidylserine.
For more apoptosis assays, review the full set of Annexin V assays, or the apoptosis assay and apoptosis marker guide.
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Platform
Flow cytometer, Fluorescence microscope
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 100 tests Annexin V-PE 1 x 500µl Binding Buffer 1 x 50ml -
Research areas
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Function
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. -
Involvement in disease
Pregnancy loss, recurrent, 3 -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
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Alternative names
- Anchorin CII
- Annexin 5
- Annexin A5
see all
Associated products
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Images
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab14155 has been referenced in 9 publications.
- Brescia M et al. High-Capacity Adenoviral Vectors Permit Robust and Versatile Testing of DMD Gene Repair Tools and Strategies in Human Cells. Cells 9:N/A (2020). PubMed: 32252479
- Xiang J et al. Melatonin-induced ApoE expression in mouse astrocytes protects endothelial cells from OGD-R induced injuries. Transl Psychiatry 10:181 (2020). PubMed: 32513932
- Tao B et al. CA10 and CA11 negatively regulate neuronal activity-dependent growth of gliomas. Mol Oncol 13:1018-1032 (2019). PubMed: 30636076
- Li S et al. HOXC10 promotes proliferation and invasion and induces immunosuppressive gene expression in glioma. FEBS J 285:2278-2291 (2018). PubMed: 29676849
- Witt AE et al. Identification of a cancer stem cell-specific function for the histone deacetylases, HDAC1 and HDAC7, in breast and ovarian cancer. Oncogene 36:1707-1720 (2017). PubMed: 27694895
- Alegret JM et al. Data on the circulating levels of endothelial microparticles are elevated in patients with bicuspid aortic valve and are related to aortic dilation. Data Brief 8:666-9 (2016). PubMed: 27453924
- Afzali M et al. Non-addictive opium alkaloids selectively induce apoptosis in cancer cells compared to normal cells. Daru 23:16 (2015). PubMed: 25890335
- Lee KS et al. nc886, a non-coding RNA of anti-proliferative role, is suppressed by CpG DNA methylation in human gastric cancer. Oncotarget 5:3944-55 (2014). PubMed: 25003254
- Stolzing A et al. Glucose-induced replicative senescence in mesenchymal stem cells. Rejuvenation Res 9:31-5 (2006). PubMed: 16608393