Annexin V-PE-Cy5 Apoptosis Staining / Detection Kit (ab14159)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnnexin V-PE-Cy5 Apoptosis Staining / Detection Kit
See all Annexin V kits -
Sample typeAdherent cells, Suspension cells
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Assay typeDirect
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Assay time0h 10m
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Product overview
Annexin V-PE-Cy5 Apoptosis Staining / Detection Kit (ab14159) is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need for fixation. Results can be analyzed by flow cytometry or fluorescence microscopy.
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Tested applicationsSuitable for: FM, Flow Cytmore details
Properties
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Storage instructionsStore at +4°C. Please refer to protocols.
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Components 100 tests Annexin V-PE 1 x 500µl Binding Buffer 1 x 50ml -
Research areas
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FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
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Involvement in diseasePregnancy loss, recurrent, 3
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Sequence similaritiesBelongs to the annexin family.
Contains 4 annexin repeats. -
DomainThe [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
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Alternative names
- Anchorin CII
- Annexin 5
- Annexin A5
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Associated products
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Applications
Our Abpromise guarantee covers the use of ab14159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| FM | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent dilution. |
Protocols
References
ab14159 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"