Anti-Annexin V/ANXA5 antibody (ab137823)
Key features and details
- Rabbit polyclonal to Annexin V/ANXA5
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Annexin V/ANXA5 antibody
See all Annexin V/ANXA5 primary antibodies -
Description
Rabbit polyclonal to Annexin V/ANXA5 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human Annexin V/ANXA5 aa 70-285.
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Positive control
- 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji and mouse brain whole cell lysates Flow cyt: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
1/50 - 1/200.
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WB |
1/500 - 1/3000. Predicted molecular weight: 35 kDa.
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Notes |
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Flow Cyt
1/50 - 1/200. |
WB
1/500 - 1/3000. Predicted molecular weight: 35 kDa. |
Target
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Function
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. -
Involvement in disease
Pregnancy loss, recurrent, 3 -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
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Database links
- Entrez Gene: 281626 Cow
- Entrez Gene: 308 Human
- Entrez Gene: 11747 Mouse
- Entrez Gene: 25673 Rat
- Omim: 131230 Human
- SwissProt: P81287 Cow
- SwissProt: P08758 Human
- SwissProt: P48036 Mouse
see all -
Alternative names
- Anchorin CII antibody
- Annexin 5 antibody
- Annexin A5 antibody
see all
Images
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All lanes : Anti-Annexin V/ANXA5 antibody (ab137823) at 1/5000 dilution
Lane 1 : WT 293T cell extracts
Lane 2 : Annexin V/ANXA5 Knockout 293T cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 35 kDa -
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Annexin V/ANXA5 using ab137823 at 1/50 dilution (red) compared to an unlabeled control (black).
A Dylight 488 secondary antibody was used.
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All lanes : Anti-Annexin V/ANXA5 antibody (ab137823) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 35 kDa
10% SDS PAGE -
Anti-Annexin V/ANXA5 antibody (ab137823) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 35 kDa
10% SDS PAGE
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab137823 has not yet been referenced specifically in any publications.