Overview

  • Product name

    Anti-Annexin V/ANXA5 antibody
    See all Annexin V/ANXA5 primary antibodies
  • Description

    Rabbit polyclonal to Annexin V/ANXA5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Annexin V/ANXA5.
    Database link: P08758

  • Positive control

    • WB: Rat PC-12 and Rat2 cell lysates; Human A431, HeLa and HepG2 cell lysates; Mouse GL261, C8D30, NIH/3T3 and RAW 264.7 cell lysates. IHC-P: Human breast carcinoma tissue.
  • General notes

     This product was previously labelled as Annexin V

     

Properties

Applications

Our Abpromise guarantee covers the use of ab140068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/1000 - 1/5000. Predicted molecular weight: 36 kDa.
IHC-P 1/500.

Target

  • Function

    This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • Involvement in disease

    Pregnancy loss, recurrent, 3
  • Sequence similarities

    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain

    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications

    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Information by UniProt
  • Database links

  • Alternative names

    • Anchorin CII antibody
    • Annexin 5 antibody
    • Annexin A5 antibody
    • Annexin V antibody
    • Annexin-5 antibody
    • ANX A5 antibody
    • ANX5 antibody
    • ANXA5 antibody
    • ANXA5_HUMAN antibody
    • Calphobindin I antibody
    • CBP-I antibody
    • Endonexin II antibody
    • ENX2 antibody
    • Lipocortin V antibody
    • PAP I antibody
    • PAP-I antibody
    • Placental anticoagulant protein 4 antibody
    • Placental anticoagulant protein I antibody
    • PLACENTAL PROTEIN 4 antibody
    • Pp4 antibody
    • Thromboplastin inhibitor antibody
    • VAC-alpha antibody
    • Vascular anticoagulant-alpha antibody
    see all

Images

  • All lanes : Anti-Annexin V/ANXA5 antibody (ab140068) at 1/50000 dilution

    Lane 1 : WT 293T cell extracts
    Lane 2 : Annexin V/ANXA5 Knockout 293T cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 36 kDa

  • Paraffin-embedded human breast carcinoma tissue stained for Annexin V/ANXA5 using ab140068 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-Annexin V/ANXA5 antibody (ab140068) at 1/5000 dilution

    Lane 1 : GL261 whole cell lysate
    Lane 2 : C8D30 whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 36 kDa

  • All lanes : Anti-Annexin V/ANXA5 antibody (ab140068) at 1/5000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 36 kDa

  • All lanes : Anti-Annexin V/ANXA5 antibody (ab140068) at 1/1000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 2 : Rat2 (rat fibroblast cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 36 kDa

References

ab140068 has not yet been referenced specifically in any publications.

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