Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Annexin V/ANXA5 antibody [EPR3979] (ab108321)

Overview

  • Product name

    Anti-Annexin V/ANXA5 antibody [EPR3979]
    See all Annexin V/ANXA5 primary antibodies
  • Description

    Rabbit monoclonal [EPR3979] to Annexin V/ANXA5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Annexin V/ANXA5 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • HeLa, JAR, and fetal kidney lysates; Human gastric tissue
  • General notes

     

     

     This product was previously labelled as Annexin V

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform antigen retrieval before commencing with IHC staining protocol

ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
    • Involvement in disease

      Pregnancy loss, recurrent, 3
    • Sequence similarities

      Belongs to the annexin family.
      Contains 4 annexin repeats.
    • Domain

      The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
      A pair of annexin repeats may form one binding site for calcium and phospholipid.
    • Post-translational
      modifications

      S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
    • Information by UniProt
    • Database links

    • Alternative names

      • Anchorin CII antibody
      • Annexin 5 antibody
      • Annexin A5 antibody
      • Annexin V antibody
      • Annexin-5 antibody
      • Annexin5 antibody
      • AnnexinA5 antibody
      • AnnexinV antibody
      • ANX 5 antibody
      • ANX A5 antibody
      • ANX5 antibody
      • ANXA5 antibody
      • ANXA5_HUMAN antibody
      • Calphobindin I antibody
      • CBP-I antibody
      • Endonexin II antibody
      • ENX 2 antibody
      • ENX2 antibody
      • Lipocortin V antibody
      • PAP I antibody
      • PAP-I antibody
      • Placental anticoagulant protein 4 antibody
      • Placental anticoagulant protein I antibody
      • PLACENTAL PROTEIN 4 antibody
      • PP 4 antibody
      • Pp4 antibody
      • RPRGL3 antibody
      • Thromboplastin inhibitor antibody
      • VAC-alpha antibody
      • Vascular anticoagulant-alpha antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: Annexin V/ANXA5 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)

      Lanes 1 - 3: Merged signal (red and green). Green - ab108321 observed at 35 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab108321 was shown to specifically react with Annexin V/ANXA5 in wild-type cells as signal was lost in Annexin V/ANXA5 knockout cells. Wild-type and Annexin V/ANXA5 knockout samples were subjected to SDS-PAGE. Ab108321 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Annexin V/ANXA5 antibody [EPR3979] (ab108321) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 36 kDa

    • Immunohistochemical analysis of Annexin V/ANXA5 in paraffin embedded Human gastric tissue, using ab108321 at a 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Campoy I  et al. Exosome-like vesicles in uterine aspirates: a comparison of ultracentrifugation-based isolation protocols. J Transl Med 14:180 (2016). Read more (PubMed: 27317346) »
    • Liu B  et al. Altered protein expression in gestational diabetes mellitus placentas provides insight into insulin resistance and coagulation/fibrinolysis pathways. PLoS One 7:e44701 (2012). WB, IHC-P ; Human . Read more (PubMed: 22970290) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
    Sample
    Human Tissue sections (human colon - muscle cells)
    Specification
    human colon - muscle cells
    Permeabilization
    Yes - 0.2% Triton X-100 and PBS
    Fixative
    Formaldehyde

    Mrs. Maria Cecilia Ybanez

    Verified customer

    Submitted Mar 11 2014

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (human colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer (10mM Sodium Citrate, 0.05% T ween 20, pH 6.0)
    Permeabilization
    Yes - 0.2% Triton X-100 and PBS
    Specification
    human colon
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Formaldehyde

    Dr. Steffen Rickelt

    Verified customer

    Submitted Nov 06 2013

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton-X100 in PBS
    Specification
    HeLa
    Fixative
    Paraformaldehyde

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Sep 04 2012

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