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Currently, I am using the abcam Annexin V antibody (ab54775) and I would like to know whether this antibody is capable to block the Annexin V (e.g. on cell surfaces). If yes, I would be interested to get some references if possibleI. If not, could you offer me a product to block Annexin V (either a blocking AV antibody or maybe a soluble phosphatidylserine).
Asked on Feb 07 2012
Thank you for contacting us and for your patience.
I have contacted the originator of anti-Annexin V antibody ab54775 and our other anti-Annexin V antibodies:
Unfortunately, it is not known whether ab54775 is capable of blocking Annexin V as this is not normally tested. Generally, it is testedif the antibody binding can be blocked, for example by using the immunogen - in this caserecombinant protein could be used to block antibody. However, we have no experience in using recombinant protein or other materials to block this antibody.
As the expression system of the recombinant protein was cell-free protein synthesis system (in vitro wheat germ protein expression system). Glycosylation and other natural eukaryotic modifications of synthesized protein do not take place in this cell-free system. Also, we are not sure whether recombinant protein will be folded correctly.
In general, we do not know whether any of our antibodies could block the Annexin V binding. The antibodies are specific for different domains of human Annexin V (for example ab108194 amino acids 296 - 320 which is in the fourth Annexin domain, http://www.uniprot.org/uniprot/P08758). If the binding to these domainsis sufficient to block Annexin V though, has not been determined yet.
Also, we currently do not have other products such as soluble phosphatidylserine in our catalogue which might be suitable for your experiments.
We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and do not want to recommend a product that may be unsuitable. We would encourage you to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about this specific research interests.
Should you decide to go ahead and test one of ourAnnexin V antibodies in blocking experiments, we encourage you to submit an Abreview of your results. We appreciate all customer feedback, whether positive or negative, and we make all product information available to researchers. To find out more about our Abreview system, please see the following webpage: https://www.abcam.com/abreviews
I am sorry that I could not be more helpful, but I hope that the available literature in this area can provide some clarification. Please do not hesitate to contact us again with other needs or with any questions about our products.
Answered on Feb 07 2012