Recombinant Anti-Annexin V/ANXA5 antibody [EPR3980] - BSA and Azide free (ab239922)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Annexin V/ANXA5 antibody [EPR3980] - BSA and Azide free
See all Annexin V/ANXA5 primary antibodies -
Description
Rabbit monoclonal [EPR3980] to Annexin V/ANXA5 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, ICC/IF, WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Annexin V/ANXA5 aa 300 to the C-terminus. The exact sequence is proprietary.
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General notes
ab239922 is the carrier-free version of ab108194 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab239922 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Annexin V
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3980 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab239922 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 36 kDa. |
Target
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Function
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. -
Involvement in disease
Pregnancy loss, recurrent, 3 -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
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Database links
- Entrez Gene: 308 Human
- Entrez Gene: 11747 Mouse
- Entrez Gene: 25673 Rat
- Omim: 131230 Human
- SwissProt: P08758 Human
- SwissProt: P48036 Mouse
- SwissProt: P14668 Rat
- Unigene: 480653 Human
see all -
Alternative names
- Anchorin CII antibody
- Annexin 5 antibody
- Annexin A5 antibody
see all
Images
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![Flow Cytometry - Anti-Annexin V/ANXA5 antibody [EPR3980] - BSA and Azide free (ab239922)](https://www.abcam.com/ps/products/239/ab239922/Images/ab239922-3-ab1081944ab108194FC.jpg)
Flow Cytometry - Anti-Annexin V/ANXA5 antibody [EPR3980] - BSA and Azide free (ab239922)Overlay histogram showing HeLa cells stained with ab108194 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108194, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108194).
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![Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody [EPR3980] - BSA and Azide free (ab239922)](https://www.abcam.com/ps/products/239/ab239922/Images/ab239922-2-AnnexinVPrimaryantibodiesab1081942.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody [EPR3980] - BSA and Azide free (ab239922) -
![Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody [EPR3980] - BSA and Azide free (ab239922)](https://www.abcam.com/ps/products/239/ab239922/Images/ab239922-1-AnnexinVPrimaryantibodiesab1081943.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody [EPR3980] - BSA and Azide free (ab239922)
Datasheets and documents
References
ab239922 has not yet been referenced specifically in any publications.
