Overview

  • Product name
    Anti-Annexin V/ANXA5 antibody [VAA-33] (FITC)
    See all Annexin V/ANXA5 primary antibodies
  • Description
    Mouse monoclonal [VAA-33] to Annexin V/ANXA5 (FITC)
  • Host species
    Mouse
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant full length protein corresponding to Human Annexin V/ANXA5.

  • General notes

     This product was previously labelled as Annexin V

     

Properties

Applications

Our Abpromise guarantee covers the use of ab63556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

Use 10µl to label 1x106 cells in 100µl.

 

 

 

ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • Involvement in disease
    Pregnancy loss, recurrent, 3
  • Sequence similarities
    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anchorin CII antibody
    • Annexin 5 antibody
    • Annexin A5 antibody
    • Annexin V antibody
    • Annexin-5 antibody
    • ANX A5 antibody
    • ANX5 antibody
    • ANXA5 antibody
    • ANXA5_HUMAN antibody
    • Calphobindin I antibody
    • CBP-I antibody
    • Endonexin II antibody
    • ENX2 antibody
    • Lipocortin V antibody
    • PAP I antibody
    • PAP-I antibody
    • Placental anticoagulant protein 4 antibody
    • Placental anticoagulant protein I antibody
    • PLACENTAL PROTEIN 4 antibody
    • Pp4 antibody
    • Thromboplastin inhibitor antibody
    • VAC-alpha antibody
    • Vascular anticoagulant-alpha antibody
    see all

References

This product has been referenced in:
  • Kosgodage US  et al. Chloramidine/Bisindolylmaleimide-I-Mediated Inhibition of Exosome and Microvesicle Release and Enhanced Efficacy of Cancer Chemotherapy. Int J Mol Sci 18:N/A (2017). Flow Cyt ; Human . Read more (PubMed: 28486412) »
  • Sacconi A  et al. miR-204 targets Bcl-2 expression and enhances responsiveness of gastric cancer. Cell Death Dis 3:e423 (2012). Flow Cyt ; Human . Read more (PubMed: 23152059) »
See all 2 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

Gracias por contactarnos.

Me temo que no tenemos en el catálogo ningún anticuerpo contra Annexin V que cumpla las características que indicas. Aquellos que están conjugados no reaccionan con humano y rata, y los que si lo hacen no están unidos a ningún fluorocromo.

Ante esta situación, puedo ofrecerte dos alternativas:

- Usar uno de los anticuerpos conjugados, no testado en rata, y participar en nuestro sistema de evaluación de anticuerpos Abtrial. Mediante este sistema, al testar un anticuerpo en una nueva aplicación o especie (siempre y cuando la homología entre secuencias sea relevante) y compartir en nuestra web los resultados, obtendrás un vale por valor del anticuerpo para una futura compra.

Para más información sobre este sistema: https://www.abcam.com/abtrial

Si te interesara participar en el programa, indícame el código del anticuerpo a testar antes de proceder a su compra.

- Conjugar vosotros mismos el anticuerpo primario que os interese, con el fluorocromo o enzima que os convenga. Para ello, tenemos unos kits de conjugación en el catálogo fáciles de utilizar, y que permiten conjugar un anticuerpo primario en poco tiempo. Para más información sobre los mismos, pincha en el siguiente https://www.abcam.com/index.html?pageconfig=resource&rid=13148.

Siento no poder ofrecerte directamente el producto deseado, pero espero que estas opciones os puedan servir a tal fin.

Si tienes cualquier otra consulta, no dudes en contactarme de nuevo.

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Answer

Thank you for email.
The problem one customer experienced with a vial of ab63556 was an isolated case and we got no signal reported. I would like to emphasize the fact that we do record all negative feedback we receive. We have provided protocol tips and have unfortunately not heard back from the customer whether these helped to solve the problem. I do not think that this case gives any indication in regards to your problem with the ab63555.
In regards to the ab79273, I just got a reply from the laboratory. Unfortunately the laboratory has not tested this antibody against any other species. Unfortunately the laboratory has not evaluated and can not make any prediction in regards to whether PS binding to lactoferrin could interfere with the epitope of the antibody.
In order to know whether the antibody crossreacts, a positive control is necessary. As mentioned in my previous email, the laboratory also recommends SKBR-3 cells. Breast, colon, or lung tissues as a positive control. Is there no means that you would obtain some in your institution to run the control?
Sorry for not being able to provide more information at this stage. I am looking forward to hear back from you in order to know how to proceed.

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Answer

Thank you very much for your email.

I can confirm that ab63556 is an antibody against Annexin V.
I am currently investigating with my colleagues how these references have come to that datasheet. I apologize for this error and thank you for pointing this error out to us. Unfortunately I am not aware of any other reference using this antibody. I would like to reassure you, that we do guarantee all our products to work as stated on the datasheet, i.e. in flow cytometry for ab63556.
If you would like to share your experience and your feedback with this antibody with others, I can recommend our Abreviews. Abreviews will allow you to rate our antibody and you will receive therefore points which are convertible into discounts or Amazon vouchers. For more information please see www.abcam.com/abreviews
I hope this information is helpful. Please do let me know if you have any other question or concern.

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Question

Dear Sir/Madam,   I completed the questionnaire. Thank you for your reply and help.     Best wishes,     Order Details: Mouse monoclonal (VAA-33) to Annexin V (FITC); ordered on 07/10/11 Antibody code: ab63556 or preferably Abcam order number: ab 63556 General Information Antibody storage conditions (temperature/reconstitution etc)  - stored in aliquots at -20C in the dark Description of the problem (high background, low signal, non-specific staining etc.) - no signal at all ample (Species/Tissue/Cell Type/Cell Line etc.) - lymphoblastoid cell line TK6; as well U2OS cells Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.) -no fixation, live cells Antigen retrieval (Enzymatic method, Heat mediated technique etc.) -non Permeabilization step -non Blocking conditions (Buffer/time period, Blocking agent etc.) -non Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step) Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step) Detection method - fluorescence microscope and in cell analyzer 2000 Positive and negative controls used (please specify) - positive control: cells treated with Ionomycin - negative control: non-treated cells Optimization attempts (problem solving) -used 1x Annexin V binding buffer (purchased from Abcam) -used different concentrations and incubation times -used different cell lines How many times have you tried the IHC? -a couple of times Have you run a "No Primary" control? -No Do you obtain the same results every time? -Yes What steps have you altered? Additional Notes            

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Answer

Thank you for taking the time to complete our questionnaire. The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality. Reviewing this case,  I would appreciate if you can confirm some further details which will help me to investigate further: 1. I am sorry to confirm that  ab63556 Annexin V antibody [VAA-33] (FITC) is not tested and guaranteed for ICC.  Regrettably, this means we would be unable to provide a free of charge replacement or credit on this occasion. I can recommend to try the antibody in flow cytometry, which has been tested and we can provide our guarantee for this. Have you tried in flow cytometry?   2. I would appreciate if you could please provide information on how you have used the primary antibody as requested on the form: Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step) 3. Please provide more details of the annexin V procedure you have used. Which products have been used?  Which catalog codes?  Has the presence of annexin V been assessed by any other methods? 4. Please provide the Abcam order reference number (or your purchase order number) and date of purchase. Thank you for your cooperation. Please do not hesitate to contact me again with the further requested details and I hope we can resolve this case for you as soon as possible.  

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Answer

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.   I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. With regards to protocols. I am contacting our original source to see if they have a copy of the testing protocol for me to send to you. I will send this on as soon as I receive it. Before deciding how to proceed, I would like to investigate this particular case further for you, and also obtain some further information for our quality monitoring and records. In order to do this, I have enclosed a questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.   We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.   Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.   Order Details Antibody code: Lot number: Purchase order number or preferably Abcam order number: General Information Antibody storage conditions (temperature/reconstitution etc) Description of the problem (high background, low signal, non-specific staining etc.) Sample (Species/Tissue/Cell Type/Cell Line etc.) Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.) Antigen retrieval (Enzymatic method, Heat mediated technique etc.) Permeabilization step Blocking conditions (Buffer/time period, Blocking agent etc.) Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step) Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step) Detection method Positive and negative controls used (please specify) Optimization attempts (problem solving) How many times have you tried the IHC? Have you run a "No Primary" control? Yes No Do you obtain the same results every time? Yes No What steps have you altered? Additional Notes We would appreciate if you are also able to provide and image which would help us to assess the results  

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Answer

Thank you for contacting us. Anti-Annexin V antibody [VAA-33] (FITC) (ab63556) is supplied in sizes applicable for 50 tests. This is equivalent to 10 μL per test. Therefore a total of 500 μL is supplied. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us. Antibody ab63556 has not been tested for reactivity with mouse annexin V or any species other than human. However, we do currently have three rabbit polyclonal antibodies that have tested positive against mouse annexin V: ab14196, ab65848, and ab90644. The first, ab14196, has been published many times and several customers have sent us positive reviews. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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