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    annexin-vanxa5-biotin-apoptosis-staining--detection-reagent-ab14165.pdf

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Cell Biology Apoptosis Annexin V Reagents
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Annexin V/ANXA5-Biotin Apoptosis Staining / Detection Reagent (ab14165)

  • Datasheet
  • SDS
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Propidium Iodide (ab14083)
ELISA
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Human Annexin V ELISA Kit (ab223863)

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Overview

  • Product name

    Annexin V/ANXA5-Biotin Apoptosis Staining / Detection Reagent
    See all Annexin V/ANXA5 reagents
  • Tested applications

    Suitable for: FM, Flow Cytmore details
  • General notes

    This product is manufactured by BioVision, an Abcam company and was previously called 1003 Annexin V-Biotin Reagent.

    Note: For more detailed information view specific protocol.

Properties

  • Form

    Liquid
  • Storage instructions

    Store at +4°C. Do not freeze.
  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Thimerosal (merthiolate)
    Constituents: 0.05% Annexin V-Biotin, 1% BSA, PBS
  • Concentration information loading...
  • Research areas

    • Cell Biology
    • Apoptosis
    • Annexin V Reagents
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Detection kits
    • Annexin V
    • Cardiovascular
    • Atherosclerosis
    • Apoptosis
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Apoptosis marker and proteins ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • Apoptosis Kits
    • Annexin V Assays
    • Annexin V-Biotin
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Annexin V
    • Annexin V Detection Kits
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Annexin V
    • Annexin V Reagents
  • Function

    This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • Involvement in disease

    Pregnancy loss, recurrent, 3
  • Sequence similarities

    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain

    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications

    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Target information above from: UniProt accession P08758 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Anchorin CII
    • Annexin 5
    • Annexin A5
    • Annexin V
    • Annexin-5
    • Annexin5
    • AnnexinA5
    • AnnexinV
    • ANX 5
    • ANX A5
    • ANX5
    • ANXA5
    • ANXA5_HUMAN
    • Calphobindin I
    • CBP-I
    • Endonexin II
    • ENX 2
    • ENX2
    • Lipocortin V
    • PAP I
    • PAP-I
    • Placental anticoagulant protein 4
    • Placental anticoagulant protein I
    • PLACENTAL PROTEIN 4
    • PP 4
    • Pp4
    • RPRGL3
    • Thromboplastin inhibitor
    • VAC-alpha
    • Vascular anticoagulant-alpha
    see all

Associated products

  • Related Products

    • Annexin V/ANXA5-FITC Apoptosis Detection Reagent (500X) (ab14082)
    • Propidium Iodide (ab14083)
    • 10X Annexin V/ANXA5 Binding Buffer (ab14084)
    • Annexin V-FITC Apoptosis Staining / Detection Kit (ab14085)
    • Annexin V-Cy3 Apoptosis Staining / Detection Kit (ab14142)
    • Annexin V/ANXA5-Cy3 Apoptosis Staining / Detection Reagent (ab14143)
    • Annexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX (ab14144)
    • Annexin V/ANXA5-Cy5 Apoptosis Staining / Detection Reagent (ab14147)
    • Annexin V-Cy5 Apoptosis Staining / Detection Kit (ab14150)
    • Annexin V-EGFP Apoptosis Staining / Detection Reagent (ab14152)
    • Annexin V-EGFP Apoptosis Staining / Detection Kit (ab14153)
    • Annexin V/ANXA5-PE Apoptosis Staining / Detection Reagent (ab14154)
    • Annexin V-PE Apoptosis Staining / Detection Kit (ab14155)
    • Annexin V-PE-Cy5 Apoptosis Staining / Detection Kit (ab14159)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab14165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
Notes
FM
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.

Protocols

  • Annexin V-Biotin Apoptosis Detection Reagent

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab14165? Please let us know so that we can cite the reference in this datasheet.

ab14165 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

For this kit, where was the Annexin-V obtained from? Is it recombinant? Or purified? And from what species?

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Abcam community

Verified customer

Asked on Dec 30 2004

Answer

The annexin V is a recombinant protein expressed from E. coli. If you have any additional questions, please contact us again.

Read More

Abcam Scientific Support

Answered on Dec 30 2004

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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