Annexin V/ANXA5-Cy3 Apoptosis Staining / Detection Reagent (ab14143)

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Overview

  • Product name

    Annexin V/ANXA5-Cy3 Apoptosis Staining / Detection Reagent
    See all Annexin V/ANXA5 reagents

  • Tested applications

    Suitable for: FM, Flow Cytmore details

  • General notes

    Annexin V/ANXA5-Cy3 Apoptosis Detection Reagent ab14143 can be used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.

    This Annexin V/ANXA5-Cy3 reagent can be used with Annexin V/ANXA5 binding buffer ab14084 (see protocol section for instructions). The Annexin V/ANXA5-Cy3 reagent is also available as part of Annexin V/ANXA5-Cy3 apoptosis kit ab14142 and Annexin V/ANXA5-Cy3 with SYTOX apoptosis kit ab14144.

    For more apoptosis assays, review the full set of Annexin V/ANXA5 assays, or the apoptosis assay and apoptosis marker guide.

Properties

Applications

Our Abpromise guarantee covers the use of ab14143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.

References

Publishing research using ab14143? Please let us know so that we can cite the reference in this datasheet.

ab14143 has been referenced in 4 publications.

  • Schuhmann D  et al. Interfering with interferon-? signalling in intestinal epithelial cells: selective inhibition of apoptosis-maintained secretion of anti-inflammatory interleukin-18 binding protein. Clin Exp Immunol 163:65-76 (2011). PubMed: 21078084
  • Thuwanut P  et al. The effects of antioxidants on semen traits and in vitro fertilizing ability of sperm from the flat-headed cat (Prionailurus planiceps). Theriogenology 76:115-25 (2011). PubMed: 21396691
  • Simonin Y  et al. Calpain activation by hepatitis C virus proteins inhibits the extrinsic apoptotic signaling pathway. Hepatology 50:1370-9 (2009). PubMed: 19711428
  • Pu P  et al. Downregulation of Wnt2 and beta-catenin by siRNA suppresses malignant glioma cell growth. Cancer Gene Ther : (2008). PubMed: 18949017

Customer reviews and Q&As

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Question

Regarding you annexin V-Cy3 conjugate, I would like to know the following: 1) What is the source of your annexin? (e.g. human placenta?) 2) Is the annexin in ab14143 purified and labeled in Abcam labs? Or is the annexin V-y3 conjugate purchased from another company and repackaged in Abcam vials? If so, which company? 3) What is the protein:dye mole ratio? 4) Where, specifically which amino acids(s), on the protein is the Cy3 dye coupled to and by which chemistry (e.g. NHS ester coupling)? 5) What method do you use to verify the binding activity of annexin V-Cy3? I also purchased annexin V-Cy3 from Sigma Aldrich and the scientist I talked to was kind enough to give me all the above information I am now requesting from you. I'm not looking to steal anyone's methods. But since we don't have time or expertise here to do annexin isolation, purification, and labeling I need to buy it. For my experiments, I require knowing the information I'm requesting above. Without this information, there are too many unknowns and I cannot use the annexin product. I look forward to your reply.

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Answer

Thank you for your enquiry. Please find below the answers to your questions. 1) The source of the annexin is human recombinant protein. 2) This product is from an originator outside of Abcam. Unfortunately, due to company policies and supplier agreements, we are not able to disclose the supplier information. 3) The protein:dye ratio is, 1 PROTEIN:4 DYE 4) The dye binds randomly to the lysine amino acid. 5) We use the annexin V-Cye3 on apoptotic Jurket cell and watch for red color expression around the cells on a flow cytometer. If there is anything else that I can help you with, please do not hesitate to contact me.

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