Annexin V/ANXA5-PE Apoptosis Staining / Detection Reagent (ab14154)

Overview

  • Product name
    Annexin V/ANXA5-PE Apoptosis Staining / Detection Reagent
    See all Annexin V/ANXA5 reagents
  • Description
    Annexin V/ANXA5-PE Apoptosis Detection Reagent
  • Tested applications
    Suitable for: FM, Flow Cytmore details
  • General notes

    Annexin V/ANXA5-PE Apoptosis Detection Reagent ab14154 can be used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.

    This Annexin V/ANXA5-PE reagent can be used with Annexin V/ANXA5 binding buffer ab14084 (see protocol section for instructions). The Annexin V/ANXA5-PE reagent is also available as part of Annexin V-PE apoptosis kit ab14155.

    For more apoptosis assays, review the full set of Annexin V/ANXA5 assays, or the apoptosis assay and apoptosis marker guide.

Properties

Applications

Our Abpromise guarantee covers the use of ab14154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.

Protocols

References

ab14154 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry. I am handling Charlottes enquiries in her absence. I have been in touch with a colleague from business development and I have been informed that the 1 x Annexin V Binding Buffer that Charlotte may have mentioned to you as being an absolute requirement for the application of Annexin V-PE Apoptosis Detection Reagent (ab14154) is now available as catalogue number ab14084: Annexin V 10X Binding Buffer (ab14084). I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry. You may keep the cells on ice for up to 30 minutes. We have not tried DAPI, but theoretically, cells can be stained with other dyes if no cell permeabilization is required. I hope this information helps. Please do not hesitate to contact us if you need anything further.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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