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    annexin-vanxa5-pe-apoptosis-staining--detection-reagent-ab14154.pdf

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Cell Biology Apoptosis Annexin V Reagents
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Annexin V/ANXA5-PE Apoptosis Staining / Detection Reagent (ab14154)

  • Datasheet
  • SDS
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Overview

  • Product name

    Annexin V/ANXA5-PE Apoptosis Staining / Detection Reagent
    See all Annexin V/ANXA5 reagents
  • Tested applications

    Suitable for: FM, Flow Cytmore details
  • General notes

    This product is manufactured by BioVision, an Abcam company and was previously called 1014 Annexin V-PE Reagent.

    Annexin V/ANXA5-PE Apoptosis Detection Reagent ab14154 can be used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.

    This Annexin V/ANXA5-PE reagent can be used with Annexin V/ANXA5 binding buffer ab14084 (see protocol section for instructions). The Annexin V/ANXA5-PE reagent is also available as part of Annexin V-PE apoptosis kit ab14155.

    For more apoptosis assays, review the full set of Annexin V/ANXA5 assays, or the apoptosis assay and apoptosis marker guide.

Properties

  • Form

    Liquid
  • Storage instructions

    Store at +4°C. Do not freeze.
  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Research areas

    • Cell Biology
    • Apoptosis
    • Annexin V Reagents
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Detection kits
    • Annexin V
    • Cardiovascular
    • Atherosclerosis
    • Apoptosis
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Apoptosis marker and proteins ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • Apoptosis Kits
    • Annexin V Assays
    • Annexin V-PE
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Annexin V
    • Annexin V Detection Kits
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Annexin V
    • Annexin V Reagents
  • Function

    This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • Involvement in disease

    Pregnancy loss, recurrent, 3
  • Sequence similarities

    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain

    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications

    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Target information above from: UniProt accession P08758 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Anchorin CII
    • Annexin 5
    • Annexin A5
    • Annexin V
    • Annexin-5
    • Annexin5
    • AnnexinA5
    • AnnexinV
    • ANX 5
    • ANX A5
    • ANX5
    • ANXA5
    • ANXA5_HUMAN
    • Calphobindin I
    • CBP-I
    • Endonexin II
    • ENX 2
    • ENX2
    • Lipocortin V
    • PAP I
    • PAP-I
    • Placental anticoagulant protein 4
    • Placental anticoagulant protein I
    • PLACENTAL PROTEIN 4
    • PP 4
    • Pp4
    • RPRGL3
    • Thromboplastin inhibitor
    • VAC-alpha
    • Vascular anticoagulant-alpha
    see all

Associated products

  • Related Products

    • Annexin V/ANXA5-FITC Apoptosis Detection Reagent (500X) (ab14082)
    • Propidium Iodide (ab14083)
    • 10X Annexin V/ANXA5 Binding Buffer (ab14084)
    • Annexin V-FITC Apoptosis Staining / Detection Kit (ab14085)
    • Annexin V-Cy3 Apoptosis Staining / Detection Kit (ab14142)
    • Annexin V/ANXA5-Cy3 Apoptosis Staining / Detection Reagent (ab14143)
    • Annexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX (ab14144)
    • Annexin V/ANXA5-Cy5 Apoptosis Staining / Detection Reagent (ab14147)
    • Annexin V-Cy5 Apoptosis Staining / Detection Kit (ab14150)
    • Annexin V-EGFP Apoptosis Staining / Detection Reagent (ab14152)
    • Annexin V-EGFP Apoptosis Staining / Detection Kit (ab14153)
    • Annexin V/ANXA5-PE Apoptosis Staining / Detection Reagent (ab14154)
    • Annexin V-PE Apoptosis Staining / Detection Kit (ab14155)
    • Annexin V-PE-Cy5 Apoptosis Staining / Detection Kit (ab14159)
    • Annexin V/ANXA5-Biotin Apoptosis Staining / Detection Reagent (ab14165)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab14154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
Notes
FM
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab14154? Please let us know so that we can cite the reference in this datasheet.

ab14154 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

I purchased from you some Annexin V-PE apoptosis detection reagent Cat. No. ab14154. I and a colleague have attempted to use this on two different types of cell lines using Flow cytometry and fluorescence microscopy all of which proved unsuccessful. We think there is something wrong with this vial of product, would it be possible to send another vial of this reagent?

Read More

Abcam community

Verified customer

Asked on Apr 03 2006

Answer

Thank you for your enquiry. I am handling Charlottes enquiries in her absence. I have been in touch with a colleague from business development and I have been informed that the 1 x Annexin V Binding Buffer that Charlotte may have mentioned to you as being an absolute requirement for the application of Annexin V-PE Apoptosis Detection Reagent (ab14154) is now available as catalogue number ab14084: Annexin V 10X Binding Buffer (ab14084). I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Apr 20 2006

Question

How long after using the kit can I store the samples at 4 degrees before I run the FACS? Can I stain with DAPI at the same time?

Read More

Abcam community

Verified customer

Asked on Dec 02 2005

Answer

Thank you for your enquiry. You may keep the cells on ice for up to 30 minutes. We have not tried DAPI, but theoretically, cells can be stained with other dyes if no cell permeabilization is required. I hope this information helps. Please do not hesitate to contact us if you need anything further.

Read More

Abcam Scientific Support

Answered on Dec 05 2005

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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