Annexin V/ANXA5-PE Apoptosis Staining / Detection Reagent (ab14154)

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Overview

  • Product name

    Annexin V/ANXA5-PE Apoptosis Staining / Detection Reagent
    See all Annexin V/ANXA5 reagents

  • Description

    Annexin V/ANXA5-PE Apoptosis Detection Reagent

  • Tested applications

    Suitable for: FM, Flow Cytmore details

  • General notes

    Annexin V/ANXA5-PE Apoptosis Detection Reagent ab14154 can be used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.

    This Annexin V/ANXA5-PE reagent can be used with Annexin V/ANXA5 binding buffer ab14084 (see protocol section for instructions). The Annexin V/ANXA5-PE reagent is also available as part of Annexin V-PE apoptosis kit ab14155.

    For more apoptosis assays, review the full set of Annexin V/ANXA5 assays, or the apoptosis assay and apoptosis marker guide.

Properties

Applications

Our Abpromise guarantee covers the use of ab14154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.

Protocols

References

ab14154 has not yet been referenced specifically in any publications.

Publishing research using ab14154? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

I purchased from you some Annexin V-PE apoptosis detection reagent Cat. No. ab14154. I and a colleague have attempted to use this on two different types of cell lines using Flow cytometry and fluorescence microscopy all of which proved unsuccessful. We think there is something wrong with this vial of product, would it be possible to send another vial of this reagent?

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Answer

Thank you for your enquiry. I am handling Charlottes enquiries in her absence. I have been in touch with a colleague from business development and I have been informed that the 1 x Annexin V Binding Buffer that Charlotte may have mentioned to you as being an absolute requirement for the application of Annexin V-PE Apoptosis Detection Reagent (ab14154) is now available as catalogue number ab14084: Annexin V 10X Binding Buffer (ab14084). I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question

How long after using the kit can I store the samples at 4 degrees before I run the FACS? Can I stain with DAPI at the same time?

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Answer

Thank you for your enquiry. You may keep the cells on ice for up to 30 minutes. We have not tried DAPI, but theoretically, cells can be stained with other dyes if no cell permeabilization is required. I hope this information helps. Please do not hesitate to contact us if you need anything further.

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