Overview

  • Product name
  • Description
    Rabbit polyclonal to Annexin VI
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Annexin VI aa 1-100. The exact sequence is proprietary.
    Database link: P08133

  • Positive control
    • IHC-P: Human L8 cells. Liver.
  • General notes

     

     

Properties

Applications

Our Abpromise guarantee covers the use of ab31026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).
IHC-P 1/50.

1/50 for 30 min at room temperature. Requires boiling of tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Target

  • Function
    May associate with CD21. May regulate the release of Ca(2+) from intracellular stores.
  • Sequence similarities
    Belongs to the annexin family.
    Contains 8 annexin repeats.
  • Domain
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications
    Phosphorylated in response to growth factor stimulation.
  • Cellular localization
    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 67 kDa calelectrin antibody
    • Annexin A6 antibody
    • Annexin VI antibody
    • Annexin VI p68 antibody
    • Annexin-6 antibody
    • AnnexinA6 antibody
    • AnnexinVI antibody
    • ANX 6 antibody
    • ANX A6 antibody
    • ANX6 antibody
    • ANXA 6 antibody
    • ANXA6 antibody
    • ANXA6_HUMAN antibody
    • Calcium binding protein p68 antibody
    • Calelectrin antibody
    • Calphobindin II antibody
    • Calphobindin-II antibody
    • CalphobindinII antibody
    • CBP 68 antibody
    • CBP68 antibody
    • Chromobindin 20 antibody
    • Chromobindin-20 antibody
    • Chromobindin20 antibody
    • CPB II antibody
    • CPB-II antibody
    • CPBII antibody
    • Lipocortin VI antibody
    • LipocortinVI antibody
    • p68 antibody
    • p70 antibody
    • Protein III antibody
    • ProteinIII antibody
    see all

Images

  • Anti-Annexin VI antibody (ab31026) at 1/500 dilution + Mouse heart tissue lysate at 50 µg

    Secondary
    HRP-conjugated Goat anti-rabbit IgG at 1/7500 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 76 kDa
    Observed band size: 76 kDa


    Exposure time: 5 seconds

    See Abreview

  • Ab31026, at a dilution of 1/50, staining formalin fixed paraffin embedded Annexin VI in human liver tissue by Immunohistochemistry.
  • All lanes : Anti-Annexin VI antibody (ab31026) at 1 µg/ml

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)
    Lane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
    Lane 4 : A498 (Human Kidney Carcinoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 76 kDa
    Observed band size: 76 kDa
    Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes
  • Anti-Annexin VI antibody (ab31026) at 1/25 dilution + lysate prepared from 293 cells

    Predicted band size: 76 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:
  • Shimura M  et al. A novel liver metastasis-correlated protein of pancreatic neuroendocrine neoplasm (PanNEN) discovered by proteomic analysis. Oncotarget 9:24291-24303 (2018). Read more (PubMed: 29849941) »
  • Spinelli C  et al. Molecular subtypes and differentiation programmes of glioma stem cells as determinants of extracellular vesicle profiles and endothelial cell-stimulating activities. J Extracell Vesicles 7:1490144 (2018). Read more (PubMed: 30034643) »
See all 11 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-15% gradient gel)
Sample
Rat Cell lysate - nuclear (rat ventricular cardiomyocyte)
Specification
rat ventricular cardiomyocyte
Treatment
1. No treatment 2. Endothelin-1 100nM 48hrs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Feb 23 2015

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Tissue lysate - whole (Heart)
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jul 26 2013

Question
Answer

I am very pleased to hear you would like to accept our offer and test ab31026 in chicken. This code will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for chicken samples and include this code in the “Additional Comments” section so we know the Abreview is for this promotion.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

We publish both positive and negative Abreviews on our datasheets so please submit the results of your tests regardless of the result.

The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Question
Answer

Thank you for contacting us. Of our Annexin VI antibodies, ab19416 is the most likely to cross-react with chicken (UniProt: P51901). The homologies of their immunogens with the chicken sequence is as follows: ab115811: 69% ab30126/ ab31100: 67% ab52221: 70% ab19416: 87% I hope this helps, please let me know if you need any additional information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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