Product nameAnti-Annexin VII antibody [203-217/6]
See all Annexin VII primary antibodies
DescriptionMouse monoclonal [203-217/6] to Annexin VII
Tested applicationsSuitable for: IHC-Fr, ICC, WBmore details
Species reactivityReacts with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human
Recombinant full length protein (Mouse)
- Whole cell extract of culture mouse connective tissue (A9) cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 1% BSA
Concentration information loading...
Purification notesPurified Immunoglobulin
Our Abpromise guarantee covers the use of ab49838 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution.
WB: Use at a concentration of 0.5 - 1.0 µg/ml. Detects a band of approximately 47 or 51 kDa depending on the tissue/cell used (predicted molecular weight: 53 kDa). A degradation product of approximately 30 kDa may also be detected.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCalcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
Tissue specificityIsoform 1 is expressed in brain, heart and skeletal muscle. Isoform 2 is more abundant in liver, lung, kidney, spleen, fibroblasts and placenta.
Sequence similaritiesBelongs to the annexin family.
Contains 4 annexin repeats.
DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
- Information by UniProt
- Annexin A7 antibody
- Annexin VII antibody
- Annexin-7 antibody
Anti-Annexin VII antibody [203-217/6] (ab49838) at 1/1000 dilution + whole cell lysate prepared from human oesophageal squamous cell carcinoma PJ41 cells at 20 µg
Goat anti-mouse IgG conjugated to HRP at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 53 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
Blocking step performed using 5% milk for 1 hour at 4°C.
ab49838 has not yet been referenced specifically in any publications.