Recombinant
RabMAb

Recombinant Anti-ANO6 antibody [EPR20910-105] (ab234422)

Overview

  • Product name

    Anti-ANO6 antibody [EPR20910-105]
    See all ANO6 primary antibodies
  • Description

    Rabbit monoclonal [EPR20910-105] to ANO6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Mouse ANO6 aa 700-850. The exact sequence is proprietary.
    Database link: Q6P9J9

  • Positive control

    • IP: Mouse spleen lysate. WB: C6, NIH/3T3 whole cell lysate. Mouse and rat spleen tissue lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20910-105
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab234422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 106 kDa.
IP 1/30.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      May act as a calcium-activated chloride channel. It is essential for calcium-dependent exposure of phosphatidylserine on the surface of activated platelets, a process necessary to trigger the clotting system.
    • Tissue specificity

      Expressed in embryonic stem cell, fetal liver, retina, chronic myologenous leukemia and intestinal cancer.
    • Involvement in disease

      Defects in ANO6 are the cause of Scott syndrome (SCOTTS) [MIM:262890]. A mild bleeding disorder due to impaired surface exposure of procoagulant phosphatidylserine (PS) on platelets and other blood cells, following activation with Ca(2+)-elevating agents.
    • Sequence similarities

      Belongs to the anoctamin family.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 2900059G15Rik antibody
      • AA407480 antibody
      • Ano6 antibody
      • ANO6_HUMAN antibody
      • Anoctamin 6 antibody
      • Anoctamin-6 antibody
      • AW554778 antibody
      • BDPLT7 antibody
      • F730003B03Rik antibody
      • MGC104751 antibody
      • SCTS antibody
      • TMEM16F antibody
      • Transmembrane protein 16F antibody
      see all

    Images

    • All lanes : Anti-ANO6 antibody [EPR20910-105] (ab234422) at 1/1000 dilution

      Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate
      Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
      Lane 3 : Mouse spleen tissue lysate
      Lane 4 : Rat spleen tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 106 kDa
      Observed band size: 120-250 kDa
      why is the actual band size different from the predicted?



      The molecular weight observed is consistent with literatures (PMID: 29507235, PMID: 26417084, PMID: 27227820, PMID: 24478309).

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure times: Lanes 1-2: 37 secs; Lane 3: 124 secs; Lane 4: 70 secs.

    • ANO6 was immunoprecipitated from 0.35 mg mouse spleen lysate with ab234422 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab234422 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
      Lane 1: Mouse spleen lysate 10 µg (Input).
      Lane 2: ab234422 IP in mouse spleen lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab234422 in mouse spleen lysate.
      Blocking/Dilution buffer: 5% NFDM/TBST.
      Exposure time: 3 mins.

      The molecular weight observed is consistent with literatures (PMID:29507235, PMID: 26417084, PMID: 27227820, PMID: 24478309)

    References

    ab234422 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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