Recombinant Anti-ANO6 antibody [EPR20910-105] (ab234422)


  • Product name

    Anti-ANO6 antibody [EPR20910-105]
    See all ANO6 primary antibodies
  • Description

    Rabbit monoclonal [EPR20910-105] to ANO6
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Mouse ANO6 aa 700-850. The exact sequence is proprietary.
    Database link: Q6P9J9

  • Positive control

    • IP: Mouse spleen lysate. WB: C6, NIH/3T3 whole cell lysate. Mouse and rat spleen tissue lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

  • Clone number

  • Isotype



Our Abpromise guarantee covers the use of ab234422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 106 kDa.
IP 1/30.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      May act as a calcium-activated chloride channel. It is essential for calcium-dependent exposure of phosphatidylserine on the surface of activated platelets, a process necessary to trigger the clotting system.
    • Tissue specificity

      Expressed in embryonic stem cell, fetal liver, retina, chronic myologenous leukemia and intestinal cancer.
    • Involvement in disease

      Defects in ANO6 are the cause of Scott syndrome (SCOTTS) [MIM:262890]. A mild bleeding disorder due to impaired surface exposure of procoagulant phosphatidylserine (PS) on platelets and other blood cells, following activation with Ca(2+)-elevating agents.
    • Sequence similarities

      Belongs to the anoctamin family.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • 2900059G15Rik antibody
      • AA407480 antibody
      • Ano6 antibody
      • ANO6_HUMAN antibody
      • Anoctamin 6 antibody
      • Anoctamin-6 antibody
      • AW554778 antibody
      • BDPLT7 antibody
      • F730003B03Rik antibody
      • MGC104751 antibody
      • SCTS antibody
      • TMEM16F antibody
      • Transmembrane protein 16F antibody
      see all


    • All lanes : Anti-ANO6 antibody [EPR20910-105] (ab234422) at 1/1000 dilution

      Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate
      Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
      Lane 3 : Mouse spleen tissue lysate
      Lane 4 : Rat spleen tissue lysate

      Lysates/proteins at 20 µg per lane.

      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 106 kDa
      Observed band size: 120-250 kDa
      why is the actual band size different from the predicted?

      The molecular weight observed is consistent with literatures (PMID: 29507235, PMID: 26417084, PMID: 27227820, PMID: 24478309).

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure times: Lanes 1-2: 37 secs; Lane 3: 124 secs; Lane 4: 70 secs.

    • ANO6 was immunoprecipitated from 0.35 mg mouse spleen lysate with ab234422 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab234422 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
      Lane 1: Mouse spleen lysate 10 µg (Input).
      Lane 2: ab234422 IP in mouse spleen lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab234422 in mouse spleen lysate.
      Blocking/Dilution buffer: 5% NFDM/TBST.
      Exposure time: 3 mins.

      The molecular weight observed is consistent with literatures (PMID:29507235, PMID: 26417084, PMID: 27227820, PMID: 24478309)


    ab234422 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab234422.
    Please use the links above to contact us or submit feedback about this product.

    For licensing inquiries, please contact

    Sign up