Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20910-105] to ANO6
- Suitable for: WB, IP
- Reacts with: Mouse, Rat
Product nameAnti-ANO6 antibody [EPR20910-105]
See all ANO6 primary antibodies
DescriptionRabbit monoclonal [EPR20910-105] to ANO6
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat
Recombinant fragment within Mouse ANO6 aa 700-850. The exact sequence is proprietary.
Database link: Q6P9J9
- IP: Mouse spleen lysate. WB: C6, NIH/3T3 whole cell lysate. Mouse and rat spleen tissue lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab234422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 106 kDa.|
FunctionMay act as a calcium-activated chloride channel. It is essential for calcium-dependent exposure of phosphatidylserine on the surface of activated platelets, a process necessary to trigger the clotting system.
Tissue specificityExpressed in embryonic stem cell, fetal liver, retina, chronic myologenous leukemia and intestinal cancer.
Involvement in diseaseDefects in ANO6 are the cause of Scott syndrome (SCOTTS) [MIM:262890]. A mild bleeding disorder due to impaired surface exposure of procoagulant phosphatidylserine (PS) on platelets and other blood cells, following activation with Ca(2+)-elevating agents.
Sequence similaritiesBelongs to the anoctamin family.
- Information by UniProt
- 2900059G15Rik antibody
- AA407480 antibody
- Ano6 antibody
All lanes : Anti-ANO6 antibody [EPR20910-105] (ab234422) at 1/1000 dilution
Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 3 : Mouse spleen tissue lysate
Lane 4 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 106 kDa
Observed band size: 120-250 kDa why is the actual band size different from the predicted?
The molecular weight observed is consistent with literatures (PMID: 29507235, PMID: 26417084, PMID: 27227820, PMID: 24478309).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-2: 37 secs; Lane 3: 124 secs; Lane 4: 70 secs.
ANO6 was immunoprecipitated from 0.35 mg mouse spleen lysate with ab234422 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab234422 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
Lane 1: Mouse spleen lysate 10 µg (Input).
Lane 2: ab234422 IP in mouse spleen lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab234422 in mouse spleen lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 mins.
The molecular weight observed is consistent with literatures (PMID:29507235, PMID: 26417084, PMID: 27227820, PMID: 24478309)
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234422 has not yet been referenced specifically in any publications.