Recombinant Anti-ANP32C/PP32R1 antibody [EPR13354-70R] (ab189921)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13354-70R] to ANP32C/PP32R1
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-ANP32C/PP32R1 antibody [EPR13354-70R]
See all ANP32C/PP32R1 primary antibodies -
Description
Rabbit monoclonal [EPR13354-70R] to ANP32C/PP32R1 -
Host species
Rabbit -
Specificity
This antibody is specific to the ANP32C/PP32R1 protein (UniProt: O43423). There is no observed cross reactivity to the Atrial Natriuretic Peptide (UniProt: P01160).
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Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- MCF-7, PC-3, BxPC-3, C6, Raw264.7 and NIH/3T3 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13354-70R -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189921 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
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IP |
1/40 - 1/60.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). |
IP
1/40 - 1/60. |
Target
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Tissue specificity
Expressed in activated stem cells, such as mobilized CD34+ cells and cord blood CD34+ cells, but not in resting bone marrow CD34+ cells. Expressed in a variety of neoplastic cell lines, mainly in prostatic adenocarcinoma cell lines. Not expressed in normal prostatic tissue. -
Sequence similarities
Belongs to the ANP32 family.
Contains 4 LRR (leucine-rich) repeats. - Information by UniProt
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Database links
- Entrez Gene: 23520 Human
- Entrez Gene: 448829 Mouse
- Entrez Gene: 680377 Rat
- Omim: 606877 Human
- SwissProt: O43423 Human
- SwissProt: Q64G17 Mouse
- Unigene: 661161 Human
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Alternative names
- Acidic (leucine rich) nuclear phosphoprotein 32 family, member C antibody
- Acidic leucine-rich nuclear phosphoprotein 32 family member C antibody
- AN32C_HUMAN antibody
see all
Images
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All lanes : Anti-ANP32C/PP32R1 antibody [EPR13354-70R] (ab189921) at 1/10000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : PC-3 cell lysate
Lane 3 : BxPC-3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa -
All lanes : Anti-ANP32C/PP32R1 antibody [EPR13354-70R] (ab189921) at 1/2000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : NIH/3T3 cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa -
Western blot analysis of ANP32C/PP32R1 in MCF-7 cell lysate immunoprecipitated using ab189921 at 1/50 dilution (Lane1). Lane 2: PBS instead of MCF-7 lysate.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab189921 has not yet been referenced specifically in any publications.