Recombinant
RabMAb

Recombinant Anti-ANP32C/PP32R1 antibody [EPR13354-70R] (ab189921)

Overview

  • Product name

    Anti-ANP32C/PP32R1 antibody [EPR13354-70R]
    See all ANP32C/PP32R1 primary antibodies
  • Description

    Rabbit monoclonal [EPR13354-70R] to ANP32C/PP32R1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ANP32C/PP32R1 aa 100-200. The exact sequence is proprietary.
    Database link: O43423

  • Positive control

    • MCF-7, PC-3, BxPC-3, C6, Raw264.7 and NIH/3T3 cell lysates.
  • General notes

     

     

     This product was previously labelled as ANP32C

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13354-70R
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab189921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
IP 1/40 - 1/60.

Target

  • Tissue specificity

    Expressed in activated stem cells, such as mobilized CD34+ cells and cord blood CD34+ cells, but not in resting bone marrow CD34+ cells. Expressed in a variety of neoplastic cell lines, mainly in prostatic adenocarcinoma cell lines. Not expressed in normal prostatic tissue.
  • Sequence similarities

    Belongs to the ANP32 family.
    Contains 4 LRR (leucine-rich) repeats.
  • Information by UniProt
  • Database links

  • Alternative names

    • Acidic (leucine rich) nuclear phosphoprotein 32 family, member C antibody
    • Acidic leucine-rich nuclear phosphoprotein 32 family member C antibody
    • AN32C_HUMAN antibody
    • ANP32 C antibody
    • ANP32C antibody
    • Phosphoprotein 32 related protein 1 antibody
    • Phosphoprotein 32-related protein 1 antibody
    • pp32 related 1 antibody
    • PP32R1 antibody
    • Tumorigenic protein pp32r1 antibody
    see all

Images

  • All lanes : Anti-ANP32C/PP32R1 antibody [EPR13354-70R] (ab189921) at 1/10000 dilution

    Lane 1 : MCF-7 cell lysate
    Lane 2 : PC-3 cell lysate
    Lane 3 : BxPC-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa

  • All lanes : Anti-ANP32C/PP32R1 antibody [EPR13354-70R] (ab189921) at 1/2000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw264.7 cell lysate
    Lane 3 : NIH/3T3 cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa

  • Western blot analysis of ANP32C/PP32R1 in MCF-7 cell lysate immunoprecipitated using ab189921 at 1/50 dilution (Lane1). Lane 2: PBS instead of MCF-7 lysate.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

References

This product has been referenced in:

  • Yang X  et al. Up-regulation of microRNA-203 inhibits myocardial fibrosis and oxidative stress in mice with diabetic cardiomyopathy through the inhibition of PI3K/Akt signaling pathway via PIK3CA. Gene 715:143995 (2019). Read more (PubMed: 31336140) »
  • Fukushima A  et al. Acetylation contributes to hypertrophy-caused maturational delay of cardiac energy metabolism. JCI Insight 3:N/A (2018). Read more (PubMed: 29769443) »
See all 7 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab189921.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up