Recombinant Anti-ANP32C/PP32R1 antibody [EPR13354-70R] - BSA and Azide free (ab251006)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13354-70R] to ANP32C/PP32R1 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-ANP32C/PP32R1 antibody [EPR13354-70R] - BSA and Azide free
See all ANP32C/PP32R1 primary antibodies -
Description
Rabbit monoclonal [EPR13354-70R] to ANP32C/PP32R1 - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody is specific to the ANP32C/PP32R1 protein (UniProt: O43423). There is no observed cross reactivity to the Atrial Natriuretic Peptide (UniProt: P01160).
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Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251006 is the carrier-free version of ab189921.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
EPR13354-70R -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
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IP |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). |
IP
Use at an assay dependent concentration. |
Target
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Tissue specificity
Expressed in activated stem cells, such as mobilized CD34+ cells and cord blood CD34+ cells, but not in resting bone marrow CD34+ cells. Expressed in a variety of neoplastic cell lines, mainly in prostatic adenocarcinoma cell lines. Not expressed in normal prostatic tissue. -
Sequence similarities
Belongs to the ANP32 family.
Contains 4 LRR (leucine-rich) repeats. - Information by UniProt
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Database links
- Entrez Gene: 23520 Human
- Entrez Gene: 448829 Mouse
- Entrez Gene: 680377 Rat
- Omim: 606877 Human
- SwissProt: O43423 Human
- SwissProt: Q64G17 Mouse
- Unigene: 661161 Human
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Alternative names
- Acidic (leucine rich) nuclear phosphoprotein 32 family, member C antibody
- Acidic leucine-rich nuclear phosphoprotein 32 family member C antibody
- AN32C_HUMAN antibody
see all
Images
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All lanes : Anti-ANP32C/PP32R1 antibody [EPR13354-70R] (ab189921) at 1/10000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : PC-3 cell lysate
Lane 3 : BxPC-3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDaThis data was developed using ab189921, the same antibody clone in a different buffer formulation.
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All lanes : Anti-ANP32C/PP32R1 antibody [EPR13354-70R] (ab189921) at 1/2000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : NIH/3T3 cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDaThis data was developed using ab189921, the same antibody clone in a different buffer formulation.
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This data was developed using ab189921, the same antibody clone in a different buffer formulation.Western blot analysis of ANP32C/PP32R1 in MCF-7 cell lysate immunoprecipitated using ab189921 at 1/50 dilution (Lane1). Lane 2: PBS instead of MCF-7 lysate. Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab251006 has not yet been referenced specifically in any publications.