Recombinant
RabMAb

Recombinant Anti-ANP32C/PP32R1 antibody [EPR13354] (ab181242)

Overview

  • Product name

    Anti-ANP32C/PP32R1 antibody [EPR13354]
    See all ANP32C/PP32R1 primary antibodies
  • Description

    Rabbit monoclonal [EPR13354] to ANP32C/PP32R1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human ANP32C/PP32R1 aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: O43423

  • Positive control

    • HUVEC, PC-3 and BxPC-3 cell lysates; MCF-7 cell lysate; PC-3 and MCF-7 cells.
  • General notes

     

     

     This product was previously labelled as ANP32C

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13354
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
ICC/IF 1/100 - 1/250.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/30 - 1/70.

Target

  • Tissue specificity

    Expressed in activated stem cells, such as mobilized CD34+ cells and cord blood CD34+ cells, but not in resting bone marrow CD34+ cells. Expressed in a variety of neoplastic cell lines, mainly in prostatic adenocarcinoma cell lines. Not expressed in normal prostatic tissue.
  • Sequence similarities

    Belongs to the ANP32 family.
    Contains 4 LRR (leucine-rich) repeats.
  • Information by UniProt
  • Database links

  • Alternative names

    • Acidic (leucine rich) nuclear phosphoprotein 32 family, member C antibody
    • Acidic leucine-rich nuclear phosphoprotein 32 family member C antibody
    • AN32C_HUMAN antibody
    • ANP32 C antibody
    • ANP32C antibody
    • Phosphoprotein 32 related protein 1 antibody
    • Phosphoprotein 32-related protein 1 antibody
    • pp32 related 1 antibody
    • PP32R1 antibody
    • Tumorigenic protein pp32r1 antibody
    see all

Images

  • All lanes : Anti-ANP32C/PP32R1 antibody [EPR13354] (ab181242) at 1/10000 dilution

    Lane 1 : HUVEC cell lysate
    Lane 2 : PC-3 cell lysate
    Lane 3 : BxPC-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1 & 3 : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
    Lane 2 : goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed PC-3 cells labeling ANP32C/PP32R1 with ab181242 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • Flow cytometric analysis of 2% paraformaldehyde-fixed MCF-7 cells labeling ANP32C/PP32R1 with ab181242 at 1/20 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Western blot analysis of ANP32C/PP32R1 in MCF-7 cell lysate immunoprecipitated with ab181242 at 1/50 dilution.

    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

References

ab181242 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181242.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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