Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13354] to ANP32C/PP32R1
- Suitable for: WB, ICC/IF, Flow Cyt, IP
- Reacts with: Human
Product nameAnti-ANP32C/PP32R1 antibody [EPR13354]
See all ANP32C/PP32R1 primary antibodies
DescriptionRabbit monoclonal [EPR13354] to ANP32C/PP32R1
Tested applicationsSuitable for: WB, ICC/IF, Flow Cyt, IPmore details
Species reactivityReacts with: Human
Recombinant fragment within Human ANP32C/PP32R1 aa 100 to the C-terminus. The exact sequence is proprietary.
Database link: O43423
- HUVEC, PC-3 and BxPC-3 cell lysates; MCF-7 cell lysate; PC-3 and MCF-7 cells.
This product was previously labelled as ANP32C
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181242 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).|
|ICC/IF||1/100 - 1/250.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/30 - 1/70.|
Tissue specificityExpressed in activated stem cells, such as mobilized CD34+ cells and cord blood CD34+ cells, but not in resting bone marrow CD34+ cells. Expressed in a variety of neoplastic cell lines, mainly in prostatic adenocarcinoma cell lines. Not expressed in normal prostatic tissue.
Sequence similaritiesBelongs to the ANP32 family.
Contains 4 LRR (leucine-rich) repeats.
- Information by UniProt
- Acidic (leucine rich) nuclear phosphoprotein 32 family, member C antibody
- Acidic leucine-rich nuclear phosphoprotein 32 family member C antibody
- AN32C_HUMAN antibody
All lanes : Anti-ANP32C/PP32R1 antibody [EPR13354] (ab181242) at 1/10000 dilution
Lane 1 : HUVEC cell lysate
Lane 2 : PC-3 cell lysate
Lane 3 : BxPC-3 cell lysate
Lysates/proteins at 20 µg per lane.
Lanes 1 & 3 : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Lane 2 : goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa
Immunofluorescent analysis of 4% paraformaldehyde-fixed PC-3 cells labeling ANP32C/PP32R1 with ab181242 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
Flow cytometric analysis of 2% paraformaldehyde-fixed MCF-7 cells labeling ANP32C/PP32R1 with ab181242 at 1/20 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Western blot analysis of ANP32C/PP32R1 in MCF-7 cell lysate immunoprecipitated with ab181242 at 1/50 dilution.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
ab181242 has not yet been referenced specifically in any publications.