Overview

  • Product name

  • Description

    Rabbit polyclonal to ANP32E
  • Host species

    Rabbit
  • Specificity

    This antibody does not cross-react with PHAPI or PHAP12a.
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human ANP32E (C terminal).
    (Peptide available as ab6242)

  • Positive control

    • A549 or HepG2 whole cell lysate (ab7900).
  • General notes

    Stable for one year.

     This product was previously labelled as LANPL

     

Properties

Applications

Our Abpromise guarantee covers the use of ab5993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 35 kDa.
IHC-P Use a concentration of 2 µg/ml.

Target

  • Function

    Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis process by modulating PP2A activity.
  • Tissue specificity

    Expressed in peripheral blood leukocytes, colon, small intestine, prostate, thymus, spleen, skeletal muscle, liver and kidney.
  • Sequence similarities

    Belongs to the ANP32 family.
    Contains 4 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
  • Post-translational
    modifications

    Phosphorylated. The phosphorylation is nuclear localization signal (NLS)-dependent.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Acidic (leucine rich) nuclear phosphoprotein 32 family member E antibody
    • Acidic leucine-rich nuclear phosphoprotein 32 family member E antibody
    • AN32E_HUMAN antibody
    • anp32e antibody
    • Cerebellar postnatal development protein 1 antibody
    • LANP like protein antibody
    • LANP-L antibody
    • LANP-like protein antibody
    • LANPL antibody
    • Leucine rich acidic nuclear protein like antibody
    • MGC5350 antibody
    see all

Images

  • Western blot analysis of PHAP III expression in human A549 (A) and HepG2 (B) cells, and rat testis (C) with anti-PHAP III at 1 µg/ml. Western blot analysis of ANP32E expression in human A549 (A) and HepG2 (B) cells, and rat testis (C) with ab5993 at 1 µg/ml.

  • ab5993 at 2µg/ml staining ANP32E in human small intestine tissue by IHC

  • ICC/IF image of ab5993 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5993, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunofluorescence of PHAP III in Human Small Intestine cells using ab5993 at 20 ug/ml.

References

This product has been referenced in:

  • Shin H  et al. Transcriptional regulation mediated by H2A.Z via ANP32e-dependent inhibition of protein phosphatase 2A. Biochim Biophys Acta 1861:481-496 (2018). CHIPseq ; Mouse . Read more (PubMed: 29524612) »
  • Mao Z  et al. Anp32e, a higher eukaryotic histone chaperone directs preferential recognition for H2A.Z. Cell Res 24:389-99 (2014). Human . Read more (PubMed: 24613878) »
See all 2 Publications for this product

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