Key features and details
- Goat polyclonal to Anterior Gradient 2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Anterior Gradient 2 antibody
See all Anterior Gradient 2 primary antibodies
DescriptionGoat polyclonal to Anterior Gradient 2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Dog
- Human colon tissue; Human small intestine tissue; Human duodenum lysate
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab115734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.01 µg/ml. Predicted molecular weight: 20 kDa.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionRequired for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth.
Tissue specificityExpressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines.
Sequence similaritiesBelongs to the AGR family.
Cellular localizationSecreted. Endoplasmic reticulum.
- Information by UniProt
- AG 2 antibody
- AG 2 protein antibody
- AG-2 antibody
ab115734 at 5ug/ml staining Anterior Gradient 2 in Human colon tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
ab115734 at 5ug/ml staining Anterior Gradient 2 in Human small intestine tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Anti-Anterior Gradient 2 antibody (ab115734) at 0.01 µg/ml + Human duodenum lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 20 kDa
Primary incubation: 1 hour
ab115734 has not yet been referenced specifically in any publications.