Recombinant Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)
![Western blot - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)](https://www.abcam.com/ps/products/76/ab76473/Images/ab76473-91912-anti-anterior-gradient-2-antibody-epr3278-western-blot.jpg "Western blot - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3278] to Anterior Gradient 2
- Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-Anterior Gradient 2 antibody [EPR3278]
See all Anterior Gradient 2 primary antibodies -
Description
Rabbit monoclonal [EPR3278] to Anterior Gradient 2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanIP HumanWB Human
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Immunogen
Synthetic peptide within Human Anterior Gradient 2 aa 1-100. The exact sequence is proprietary.
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Positive control
- HT-29 and MCF-7 cell lysates. Human colon tissue. Human endometrium adenocarcinoma. ICC/IF: MCF7 cells Flow Cyt: HT-29 cells IP: HT-29 whole cell lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3278 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| IP |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000 - 1/10000. Predicted molecular weight: 20 kDa.
|
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| IP |
1/20.
|
|
| IHC-P | (1) |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Flow Cyt |
1/40.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF |
1/100.
|
| Notes |
|---|
|
WB
1/5000 - 1/10000. Predicted molecular weight: 20 kDa. |
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IP
1/20. |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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Flow Cyt
1/40. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF
1/100. |
Target
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Function
Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. -
Tissue specificity
Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines. -
Sequence similarities
Belongs to the AGR family. -
Cellular localization
Secreted. Endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 10551 Human
- Omim: 606358 Human
- SwissProt: O95994 Human
- Unigene: 530009 Human
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Alternative names
- AG 2 antibody
- AG 2 protein antibody
- AG-2 antibody
see all
Images
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Western blot - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)All lanes : Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473) at 1/10000 dilution
Lane 1 : HT-29 cell lysates
Lane 2 : MCF-7 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 20 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)](https://www.abcam.com/ps/products/76/ab76473/Images/ab76473-4-anti-anterior-gradient-2-antibody-epr3278-immunocytochemistry-mcf7-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling Anterior Gradient 2 with purified ab76473 at 1/100 dilution (3.5 µg/mL). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)](https://www.abcam.com/ps/products/76/ab76473/Images/ab76473-91911-anti-anterior-gradient-2-antibody-epr3278-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)ab76473, at 1/500 dilution, staining Anterior Gradient 2 in paraffin-embedded human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)](https://www.abcam.com/ps/products/76/ab76473/Images/ab76473-7-anti-anterior-gradient-2-antibody-epr3278-immunoprecipitation-ht29-human.jpg)
Immunoprecipitation - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)Purified ab76473 at 1/20 dilution (2µg) immunoprecipitating Anterior Gradient 2 in HT-29 whole cell lysate.
Lane 1 (input): HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab76473 + HT-29 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76473 in HT-29 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 17 kDa -
![Flow Cytometry - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)](https://www.abcam.com/ps/products/76/ab76473/Images/ab76473-6-anti-anterior-gradient-2-antibody-epr3278-flowcytometry-ht29-human.jpg)
Flow Cytometry - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)Flow Cytometry analysis of HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labeling Anterior Gradient 2 with purified ab76473 at 1/40 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)](https://www.abcam.com/ps/products/76/ab76473/Images/ab76473-91910-anti-anterior-gradient-2-antibody-epr3278-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)ab76473, at 1/500 dilution, staining Anterior Gradient 2 in paraffin-embedded human endometrium adenocarcinoma by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)](https://www.abcam.com/ps/products/76/ab76473/Images/ab76473-5-benefits-of-recombinant-antibodies.png)
Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)
Protocols
Datasheets and documents
References (12)
ab76473 has been referenced in 12 publications.
- Yang J et al. Long Noncoding RNA LINC00460 Promotes Hepatocellular Carcinoma Progression via Regulation of miR-342-3p/AGR2 Axis. Onco Targets Ther 13:1979-1991 (2020). PubMed: 32184630
- Dahal Lamichane B et al. AGR2 is a target of canonical Wnt/ß-catenin signaling and is important for stemness maintenance in colorectal cancer stem cells. Biochem Biophys Res Commun 515:600-606 (2019). PubMed: 31178140
- Broustas CG et al. RAD9A promotes metastatic phenotypes through transcriptional regulation of anterior gradient 2 (AGR2). Carcinogenesis 40:164-172 (2019). PubMed: 30295739
- Karagianni AE et al. Transcriptional Response of Ovine Lung to Infection with Jaagsiekte Sheep Retrovirus. J Virol 93:N/A (2019). PubMed: 31434729
- Li X et al. A qualitative transcriptional signature for the histological reclassification of lung squamous cell carcinomas and adenocarcinomas. BMC Genomics 20:881 (2019). PubMed: 31752667
