Product nameAntibody Barcoding kit - 5' Series - A001-A010 - Lightning-Link®
This technology works by targeting amine groups (e.g. lysines) and is widely used to label antibodies. Most oligo conjugation protocols are complex and time-consuming, often resulting in low antibody recovery. Antibody Barcoding kit - 5' Series - A001-A010 - Lightning-Link® (ab270703) allow reactions of antibodies and barcoded oligos to be set up in less than 30 seconds, simply by adding a solution of each antibody to lyophilized vials each containing a different pre-activated barcoded oligo.
Since separate antibody activation and oligo activation steps are not required, 10 μg scale conjugations can be carried out with ease.
A proprietary optional clean-up module for removing free oligo is also provided in the kit.
This product is manufactured by Expedeon, an Abcam company. It was previously called Lightning-Link® Antibody Barcoding kit - 5' Series (10x10 μg - A001-A010). Expedeon product code C-A0010 is the same as the 1 kit size of this product.
For oligo sequence information please see appendix in protocol booklet.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 kit Barcode Modifier 1 vial Conjugate Resuspension Buffer 1 vial Oligo A001 1 vial Oligo A002 1 vial Oligo A003 1 vial Oligo A004 1 vial Oligo A005 1 vial Oligo A006 1 vial Oligo A007 1 vial Oligo A008 1 vial Oligo A009 1 vial Oligo A010 1 vial Quencher N 1 vial Reagent C 1 vial Reagent S 1 vial
Antibody Barcoding kit - 5' Series - A001-A010 - Lightning-Link® (ab270703) enables the rapid and simple conjugation of antibodies of choice to barcoded oligonucleotides designed for Genomic Cytometry techniques.
- X represents the nucleotides of the barcode, each sequence is unique to these kits.
- N represents a randomly selected A, C, G, or T.
- The symbol * indicates a phosphorothioated bond, this modification renders the internucleotide linkage resistant to nuclease degradation.
ab270703 has not yet been referenced specifically in any publications.