Product nameAntibody Biotinylation Check Kit
Antibody Biotinylation Check Kit (ab270030) is a quick immunochromatography test that allows you to confirm the successful conjugation of biotin to your antibody without the need for any specialized or costly equipment. Furthermore, this qualitative lateral flow assay has the great advantage of being simpler and quicker compared to the HABA assay, traditionally used for biotinylation detection.
Furthermore, this qualitative lateral flow assay has the great advantage of being simpler and quicker compared to the HABA assay, traditionally used for biotinylation detection.
This product is manufactured by Expedeon, an Abcam company. It was previously called Biotin Check&Go! Antibody Labeling Confirmation Kit. Expedeon product code 4001-0030 is the same as the 30 test size of this product.
The key component of the kit is a nitrocellulose membrane containing a Test line of immobilized Protein A and Protein G called a half strip. Both Protein A and Protein G have a high affinity for the Fc region of a variety of IgG molecules. The half strips also contain an absorbent pad to promote and control the flow of sample through the nitrocellulose. The biotinylated antibody is run on the Protein A/G Strip together with 40 nm gold conjugated streptavidin as the detection label. Biotin binds to streptavidin with exceptionally high affinity and when the biotinylated antibody flows along the nitrocellulose, upon binding to the Protein A and Protein G concentrated on the Test line, a visible red line appears on the strip.
Storage instructionsPlease refer to protocols.
Components 30 tests 10X Running Buffer 2 vials 40nm gold conjugated streptavidin (Lyophilized) 1 vial Biotinylated Rabbit IgG (Lyophilized) 1 vial Protein A/G Strips 30 tests
ab270030 has not yet been referenced specifically in any publications.