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Customer kindly contacted us as they had excessive antibody loss during buffer exchange and labeling. To help with their troubleshooting of this, they where hoping that we could explain the use of PEG and sucrose as buffer for this AB as compared with other stabilizers such as BSA. Is this product sticky so that during buffer exchange it may aggregate?
Asked on Oct 12 2012
Thank you for contacting us regarding ab58555.
I have contacted the lab on your behalf, the antibody would be fine as well in a different buffer containing another stabilizing agent e.g. BSA. However this product is provided in PEG and sucrose as was found the antibody was suffering less during lyophilization.
The antibody itself is not stickier than other antibodies. It seems possible that the buffer with the PEG and sucrose is responsible of the low yield that you are getting after the conjugation. The buffer exchange method may need to be adjusted for improved performance.
We would recommend use of one of our concentration kits for performing buffer exchange with this product. Our antibody concentration and clean up kits, Ab102776 and ab102778utilizes a simple spin column to easily and quickly buffers. These should work in any buffer containing PEG and are covered by our Abpromise guarantee. Which guarantees scientific support, replacement or refund should any Abcam product not perform as indicated on the datasheet. More information about Ab102776, ab102778 and our Abpromise may be found at the link below.
I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.
Answered on Oct 12 2012