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Customer contacted us regarding the use of the antibody concentration kit (ab102778) to concentrate 1ml of a 30kDa Fab fragment solution into 50-100uL. Both antibody concentration and buffer exchange procedures are not yielding increased concentration - measure by nanodrop at OD280 before and after concentration protocols are performed.
Asked on Jan 17 2013
The laboratory responded that the filter in the antibody concentration kit ab102778 should work fine with a 30KD fragment. It is possible that the filter could be damaged. It would help if you could measure the OD of the flow through. If the flow through has the same OD then there is a problem with the filter. If not, then there may be an issue with the OD measurements, perhaps a blanking issue or issue with the reader itself.
Please let me know the results of the OD readings of the flow through. I look forward to your reply.
Answered on Jan 17 2013