Question (76183) | Antibody Concentration Kit (ab102778)

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Question

I plan to concentrate 30-40kDa Fab solution with Antibody Concentration Kit (ab102778). May I ask the mechanism of this Kit? Will the small size molecules be passed through the filter during centrifugation? Will FITC (400˜Da) be passed through?

Answer


The Antibody Concentration Kit method utilises a simple spin column to easily and quickly remove excess buffer from the antibody thereby providing a more concentrated antibody solution. Only molecules smaller than 10 kDa will be pass through the filter. Therefore, FITC alone will pass through the filter, unless it is conjugated to a molecule larger than 10 kDa, such as the antibody that you are concentrating.

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