For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
I plan to concentrate 30-40kDa Fab solution with Antibody Concentration Kit (ab102778). May I ask the mechanism of this Kit? Will the small size molecules be passed through the filter during centrifugation? Will FITC (400˜Da) be passed through?
Asked on Sep 25 2014
The Antibody Concentration Kit method utilises a simple spin column to easily and quickly remove excess buffer from the antibody thereby providing a more concentrated antibody solution. Only molecules smaller than 10 kDa will be pass through the filter. Therefore, FITC alone will pass through the filter, unless it is conjugated to a molecule larger than 10 kDa, such as the antibody that you are concentrating.
Guillaume BoucherAbcam Scientific Support
Answered on Sep 25 2014