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Customer wants to purify mouse monoclonal IgG that is in PBS and azide. Which kit to use?
Asked on Mar 04 2015
Thank you for your call and your patience while I communicated with my colleague in the lab.
In response to your inquiry, it turns out that both options (ab102784 Antibody Purification Kit (Protein A) or ab102778 Antibody Concentration Kit ) will work for your antibody purification purposes. However it turns out that the simplest kits would be the Concentration kit.
The spin columns provided in this kit allow a simple buffer exchange by ‘spinning away’ the existing buffer, and allowing this to be replaced by a more compatible buffer. As spinning the antibody dry is not best practice, as this can impact the antibody, the protocol states that this allows the azide to be reduced, as there will be a small amount of azide left in the little bit of buffer that remains. However if this process is repeated, each time, the amount of azide remaining will reduce more and more, therefore after enough spins, the azide concentration will effectively become zero. On the whole, this azide should be reduced to a suitable level almost after 1 buffer exchange, however we would recommend this is repeated further if the label of choice is HRP, as this has shown to be more sensitive. I hope you find this information helpful.
Please let me know if I can offer any more help or technical advice. Good luck with your research.
Answered on Mar 06 2015