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I want to purify 500 ul of rabbit serum and do you think such a small amount would work ok with this kit as I was worried about diluting my antibody so much. Or could you recommend an alternative?
Asked on Oct 03 2014
It is true that in the protocol we recommend using 1.3-1.7ml of rabbit IgG.
0.5ml volume is therefore too low to use with this purification kit, so we would recommend diluting the antibody in a binding buffer to get a volume of 1.3ml and continuing with the protocol as normal . We do not see any reason why diluting the antibody further would affect their downstream process’ as there is an antibody concentration step at the end the protocol.
Elisa ThomasAbcam Scientific Support
Answered on Oct 03 2014