Question (76202) | Antibody Serum Purification Kit (Protein A) (ab109209)

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Question

I want to purify 500 ul of rabbit serum and do you think such a small amount would work ok with this kit as I was worried about diluting my antibody so much. Or could you recommend an alternative?

Answer

It is true that in the protocol we recommend using 1.3-1.7ml of rabbit IgG.

0.5ml volume is therefore too low to use with this purification kit, so we would recommend diluting the antibody in a binding buffer to get a volume of 1.3ml and continuing with the protocol as normal . We do not see any reason why diluting the antibody further would affect their downstream process’ as there is an antibody concentration step at the end the protocol.

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