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Antigen Retrieval Buffer (100X Citrate Buffer pH 6.0) (ab93678)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Citrate Buffer pH 6.0) (ab93678)

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    Overview

    • Product name

      Antigen Retrieval Buffer (100X Citrate Buffer pH 6.0)
    • Tested applications

      Suitable for: IHC-Pmore details
    • General notes

      Antigen Retrieval Buffer (100X Citrate Buffer pH 6.0) is a special solution that enables rehydration and antigen retrieval to be performed in formalin-fixed, paraffin-embedded tissue sections with one step. It is optimal for use with primary antibodies that require citrate buffer (pH 6.0) pretreatment.

      This product contains detergent for emulsification of the paraffin.

      1X Dilution: The 100X stock solution should be diluted 100-fold with distilled water before use.

      Protocol:
      1. Place paraffin-embedded slides in 1x Antigen Retrieval Buffer; cover with a vented plastic wrap, place in microwave and set high power to boil and then set low power to keep it boiling for 10 min. Let the sections cool in the microwave for at least 20 min.
      2. Wash sections with hot tap water for 1 min.
      3. Wash sections in buffer for 2 x 3 minutes.
      4. Continue with IHC protocol as normal.

      Other kits and reagents for IHC

      Other antigen retrieval buffers include: Tris-EDTA buffer pH 9.0 ab93684, EDTA buffer pH 8.0 ab93680, Tris buffer pH 10.0 ab93682, or see the full list of antigen retrieval buffers and enzymes.

      Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

    Properties

    • Form

      Liquid
    • Storage instructions

      Store at room temperature.
    • Storage buffer

      pH: 5.1
      Constituents: Citrate Buffer, 1% Hydrochloric acid, 1.5% 2-butoxyethanol

      Buffer 100x Concentrated with detergent.
    • Concentration information loading...
    • Research areas

      • Kits/ Lysates/ Other
      • Tools and Reagents
      • IHC Tools/ Reagents
      • Kits/ Lysates/ Other
      • Kits
      • IHC tools
      • Antigen retrieval reagent
      • Other
    • Relevance

      Antigen Retrieval Buffer (100X Citrate Buffer pH 6.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. It is optimal for use with primary antibodies that require citrate buffer (pH=6.0) pretreatment.

    Associated products

      Applications

      Our Abpromise guarantee covers the use of ab93678 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Abreviews Notes
      IHC-P Use at an assay dependent dilution.

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Citrate Buffer pH 6.0) (ab93678)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Citrate Buffer pH 6.0) (ab93678)
        ab93678 Antigen Retrieval Bufer 100X Citrate Buffer pH 6

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (6)

      Publishing research using ab93678? Please let us know so that we can cite the reference in this datasheet.

      ab93678 has been referenced in 6 publications.

      • Roberts RF  et al. Alpha-Synuclein Proximity Ligation Assay (AS-PLA) in Brain Sections to Probe for Alpha-Synuclein Oligomers. Methods Mol Biol 1948:69-76 (2019). PubMed: 30771171
      • Racioppi L  et al. CaMKK2 in myeloid cells is a key regulator of the immune-suppressive microenvironment in breast cancer. Nat Commun 10:2450 (2019). PubMed: 31164648
      • Ferreira LL  et al. The effect of dental bleaching on pulpal tissue response in a diabetic animal model: a study of immunoregulatory cytokines. Int Endod J 51:347-356 (2018). PubMed: 28857196
      • Kageyama Y  et al. Amyloid ß toxic conformer has dynamic localization in the human inferior parietal cortex in absence of amyloid plaques. Sci Rep 8:16895 (2018). PubMed: 30442978
      • McClurg UL  et al. Ex vivo Culture and Lentiviral Transduction of Benign Prostatic Hyperplasia (BPH) Samples. Bio Protoc 8:N/A (2018). PubMed: 30515449
      • Doucet M  et al. Preclinical development of a vaccine against oligomeric alpha-synuclein based on virus-like particles. PLoS One 12:e0181844 (2017). PubMed: 28797124

      Customer reviews and Q&As

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      Antigen Retrieval Buffer

      Excellent Excellent 5/5 (Ease of Use)
      Abreviews
      Abreviews
      We used the buffer on paraffin samples. The working concentration of the buffer is 1x and we used it in the steam pot. The buffer works very well and is easy to use.

      Abcam user community

      Verified customer

      Submitted Aug 29 2019

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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