Overview

  • Product name

    Antigen Retrieval Buffer (100X Citrate Buffer pH 6.0)
  • Tested applications

    Suitable for: IHC-Pmore details
  • General notes

    Antigen Retrieval Buffer (100X Citrate Buffer pH 6.0) is a special solution that enables rehydration and antigen retrieval to be performed in formalin-fixed, paraffin-embedded tissue sections with one step. It is optimal for use with primary antibodies that require citrate buffer (pH 6.0) pretreatment.

    This product contains detergent for emulsification of the paraffin.

    1X Dilution: The 100X stock solution should be diluted 100-fold with distilled water before use.

    Protocol:
    1. Place paraffin-embedded slides in 1x Antigen Retrieval Buffer; cover with a vented plastic wrap, place in microwave and set high power to boil and then set low power to keep it boiling for 10 min. Let the sections cool in the microwave for at least 20 min.
    2. Wash sections with hot tap water for 1 min.
    3. Wash sections in buffer for 2 x 3 minutes.
    4. Continue with IHC protocol as normal.

    Other kits and reagents for IHC

    Other antigen retrieval buffers include: Tris-EDTA buffer pH 9.0 ab93684EDTA buffer pH 8.0 ab93680Tris buffer pH 10.0 ab93682, or see the full list of antigen retrieval buffers and enzymes.

    Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Properties

  • Form

    Liquid
  • Storage instructions

    Store at room temperature.
  • Storage buffer

    pH: 6.00
    Constituents: Citrate Buffer, 1.5% 2-butoxyethanol, 1% Hydrochloric acid
    Note: Buffer 100x Concentrated with detergent.
  • Concentration information loading...
  • Research areas

  • Relevance

    Antigen Retrieval Buffer (100X Citrate Buffer pH 6.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. It is optimal for use with primary antibodies that require citrate buffer (pH=6.0) pretreatment.

Applications

Our Abpromise guarantee covers the use of ab93678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

Images

  • ab93678 Antigen Retrieval Bufer 100X Citrate Buffer pH 6

References

This product has been referenced in:

  • Roberts RF  et al. Alpha-Synuclein Proximity Ligation Assay (AS-PLA) in Brain Sections to Probe for Alpha-Synuclein Oligomers. Methods Mol Biol 1948:69-76 (2019). Read more (PubMed: 30771171) »
  • Ferreira LL  et al. The effect of dental bleaching on pulpal tissue response in a diabetic animal model: a study of immunoregulatory cytokines. Int Endod J 51:347-356 (2018). Read more (PubMed: 28857196) »
See all 5 Publications for this product

Customer reviews and Q&As

Filter by Ratings

Antigen Retrieval Buffer

Excellent Excellent 5/5 (Ease of Use)
Abreviews
We used the buffer on paraffin samples. The working concentration of the buffer is 1x and we used it in the steam pot. The buffer works very well and is easy to use.

Abcam user community

Verified customer

Submitted Aug 29 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up