Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) (ab93680)
Overview
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Product name
Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) -
Tested applications
Suitable for: IHC-Pmore details -
General notes
Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. It is optimal for use with primary antibodies that require EDTA buffer (pH=8.0) pretreatment.
This product contains detergent for emulsification of the paraffin.
1X Dilution: The 100X stock solution should be diluted 100-fold with distilled water before use.
PROTOCOL:
- Place paraffin-embedded slides in 1x Antigen Retrieval Buffer; cover with a vented plastic wrap, place in microwave and set high power to boil and then set low power to keep it boiling for 10 min. Let the sections cool in the microwave for at least 20min.
- Wash sections with hot tap water for 1 minute.
- Wash sections in buffer for 2x3 minutes.
- Continue with IHC protocol as normal.
Other kits and reagents for IHC
Other antigen retrieval buffers include:
Citrate buffer pH 6.0 ab93678, Tris-EDTA buffer pH 9.0 ab93684, Tris buffer pH 10.0 ab93682, or see the full list of antigen retrieval buffers and enzymes.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Properties
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Form
Liquid -
Storage instructions
Store at room temperature. -
Storage buffer
pH: 8
Constituents: 1.5% 2-butoxyethanol, 5% Sodium EDTA
Buffer 100x Concentrated with detergent. -
Concentration information loading... -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent dilution.
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| Notes |
|---|
|
IHC-P
Use at an assay dependent dilution. |
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) (ab93680)Image from Wang et al., Nat Commun., 11(1):220; doi: 10.1038/s41467-019-14046-9. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/Prolonged survival of Lgr5/Apc and MYC-ON mice depends on the T cells.g Immunohistochemical staining of GFP in the indicated genotypes (n = 5 mice per group).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) (ab93680)Image from Chao-Wei et al., BMC Complement Altern Med., 6(1):310; doi: 10.1186/s12906-016-1278-7. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/Ophiocordyceps formosana upregulated adiponectin expressions in plasma and adipose tissuesb) Immunohistochemical staining for adiponectin in adipose tissues. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) (ab93680)ab93680 Antigen Retrieval Buffer 100X EDTA Buffer, pH 8
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab93680 has been referenced in 4 publications.
- Wang Y et al. Tissue-resident macrophages promote extracellular matrix homeostasis in the mammary gland stroma of nulliparous mice. Elife 9:N/A (2020). PubMed: 32479261
- Wang G et al. Arf1-mediated lipid metabolism sustains cancer cells and its ablation induces anti-tumor immune responses in mice. Nat Commun 11:220 (2020). PubMed: 31924786
- Huang CW et al. Ophiocordyceps formosana improves hyperglycemia and depression-like behavior in an STZ-induced diabetic mouse model. BMC Complement Altern Med 16:310 (2016). IHC-P . PubMed: 27553852
- Zhu Y et al. Elevated expression of AKT2 correlates with disease severity and poor prognosis in human osteosarcoma. Mol Med Rep 10:737-42 (2014). PubMed: 24919955