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    antigen-retrieval-buffer-100x-tris-buffer-ph-100-ab93682.pdf

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Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0) (ab93682)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0) (ab93682)

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    Overview

    • Product name

      Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0)
    • Tested applications

      Suitable for: IHC-Pmore details
    • General notes

      Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. It is optimal for use with primary antibodies that require Tris buffer (pH=10.0) pretreatment.

      This product contains detergent for emulsification of the paraffin.

      1X Dilution: The 100X stock solution should be diluted 100-fold with distilled water before use.

      PROTOCOL:

      1. Place slides in a Coplin jar containing 1x Target Retrieval Buffer; cover with a vented plastic wrap and place the jar in microwave and set high power to boil and set low power to keep it boiling for 10 min. Let the sections cool in the microwave for at least 20min.
      2. Wash sections with hot tap water for 1 minute.
      3. Wash sections in buffer for 2x3 minutes.
      4. Block endogenous peroxidase as usual.
      5. Wash sections in buffer for 2x3 minutes.
      6. Block non-specific sites with a Blocking reagent as usual.
      7. Place optimally diluted primary antibody on the sections (incubation time and temperature for a given set of experimental conditions should be determined by the investigator).
      8. Wash sections in buffer for 2x3 minutes
      9. Rest of the procedure is same as routinely performed in your laboratory.

      Other kits and reagents for IHC

      Other antigen retrieval buffers include: Citrate buffer pH 6.0 ab93678, EDTA buffer pH 8.0 ab93680, Tris-EDTA buffer pH 9.0 ab93684 or see the full list of antigen retrieval buffers and enzymes.

      Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

    Properties

    • Form

      Liquid
    • Storage instructions

      Store at room temperature.
    • Storage buffer

      pH: 11
      Constituents: 98.5% Tris buffer, 1.5% 2-butoxyethanol

      Buffer 100x concentrated with detergent.
    • Concentration information loading...
    • Research areas

      • Kits/ Lysates/ Other
      • Tools and Reagents
      • IHC Tools/ Reagents
      • Kits/ Lysates/ Other
      • Kits
      • IHC tools
      • Antigen retrieval reagent
      • Other

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab93682 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Use at an assay dependent dilution.
    Notes
    IHC-P
    Use at an assay dependent dilution.

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0) (ab93682)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0) (ab93682)
      ab93682 Antigen Retrieval Buffer 100X Tris Buffer, pH 10

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab93682? Please let us know so that we can cite the reference in this datasheet.

    ab93682 has not yet been referenced specifically in any publications.

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