Overview

  • Product name
    Anti-Antithrombin III / ATIII antibody
    See all Antithrombin III / ATIII primary antibodies
  • Description
    Sheep polyclonal to Antithrombin III / ATIII
  • Host species
    Sheep
  • Tested applications
    Suitable for: Double Immunodiffusion, RID, Immunoelectrophoresismore details
  • Species reactivity
    Reacts with: Mouse, Rat, Horse, Cat, Dog, Human
    Does not react with: Rabbit, Chicken, Guinea pig, Cow, Pig
  • Immunogen

    Full length native protein (purified) corresponding to Human Antithrombin III/ ATIII. Purified from Plasma

  • General notes
    The concentration varies from batch to batch (10-20 mg/mL).

    Previously labelled as Antithrombin III.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 0.1% EACA, 0.01% Benzamidine, 0.0292% EDTA
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Antiserum is prepared by immunisation of sheep with Antithrombin III / ATIII and, if necessary, adsorbed to monospecificity by use of solid-phase adsorbents. An immunoglobulin fraction is then produced. The titre is adjusted so that inter-batch variation is within 10%. The product is 0.2µm filtered.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Double Immunodiffusion
RID
Rocket Immunoelectrophoresis
Immunoelectrophoresis
  • Application notes
    RID and Rocket IEP: 6µL antiserum/cm² gel vs 5µL neat – 1/10 dilution human plasma
    Double diffusion: 10µL antiserum vs 5µL plasma
    IEP: 100µL antiserum vs 5µL plasma
    The use of 3% PEG 6000 with 1.2%agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended.
    Suitability for use in nephelometry, immunohistochemical procedures, enzyme-linked immunosorbentassays and Western blot has not been assessed but use in such assays should not necessarily beexcluded.
  • Target

    • Function
      Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
    • Tissue specificity
      Found in plasma.
    • Involvement in disease
      Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations.
    • Sequence similarities
      Belongs to the serpin family.
    • Post-translational
      modifications
      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization
      Secreted > extracellular space.
    • Information by UniProt
    • Database links
    • Alternative names
      • ANT3_HUMAN antibody
      • Antithrombin antibody
      • Antithrombin III antibody
      • Antithrombin-III antibody
      • AntithrombinIII antibody
      • AT 3 antibody
      • AT III antibody
      • AT3 antibody
      • AT3D antibody
      • ATIII antibody
      • Heparin cofactor I antibody
      • MGC22579 antibody
      • Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1 antibody
      • Serine cysteine proteinase inhibitor clade C member 1 antibody
      • Serine proteinase inhibitor clade C member 1 antibody
      • Serpin C1 antibody
      • Serpin family C member 1 antibody
      • Serpin peptidase inhibitor clade C (antithrombin) member 1 antibody
      • SERPINC1 antibody
      • THPH7 antibody
      see all

    References

    ab8776 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    I have received the following protocol for ab13335 in direct ELISA (not sandwich as sandwich ELISA has not been tested with ab13335) which I hope will help you. 1. Coating 1-5 ug/ml per well of Anti-thrombin III on High binding plates. Incubation for overnight at 4C. 2. Blocking with 1% BSA in PBS for 30 minutes. 3. Wash buffer: PBS Tween 0.05%. 4. Primary antibody: this product at 1:1,000-1:2,000 dilutions. Incubation for 120 minutes at room temperature. 5. Secondary antibody: HRP APA mouse IgG at 1:500-1:1,000 dilutions. Incubation for 120 minutes at room temperature. 6. Substrate: OPD Please let me know if this helps. Best of luck with your research

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    Answer

    Thank you for your email. To our knowledge, this antibody has yet to be tested in Western blotting. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. If you have any additional questions, please contact us again.

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    Question
    Answer

    Thank you for your enquiry. We are sorry to inform you that there is no protein concentration for polyclonal antibodies that are not affinity purified. If you have any more questions, please contact us again.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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